肝细胞癌及癌旁肝组织中bcl-2和p53蛋白表达与端粒酶活性的相关性研究  

Relationship among bcl 2 and p53 protein expressions and telomerase activity in hepatocellular carcinomas and their surrounding liver tissue

作　　者：冯德云[1] 郑晖[1] 程瑞雪[1] 傅春燕[1] 

机构地区：[1]湖南医科大学病理学教研室,长沙410078

出　　处：《临床与实验病理学杂志》1999年第4期287-289,I043,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：卫生部基金

摘　　要：目的：探讨ｂｃｌ２ 和ｐ５３ 蛋白的表达与端粒酶活性的相关性及其与 Ｈ Ｃ Ｃ 发生的关系。方法：利用端粒酶原位标记法显示端粒酶活性，采用 Ｓ Ｐ 法免疫组化技术检测ｂｃｌ２ 和ｐ５３ 蛋白。结果：端粒酶在 Ｈ Ｃ Ｃ 中的阳性率（９１７ ％ ） 显著高于癌旁肝组织（５８３ ％ ）（ Ｐ＜ ００５） ，端粒酶活性强度与 Ｈ Ｃ Ｃ 分化程度无关（ Ｐ＞ ００５） ；癌组织中ｂｃｌ２ 和ｐ５３ 蛋白的阳性率均高于癌旁组织（ Ｐ＜ ００１） ； Ｈ Ｃ Ｃ 和癌旁组织中端粒酶活性程度随ｂｃｌ２ 蛋白表达增强而升高，并呈明显正相关，但与ｐ５３ 蛋白表达强度无明显相关性。结论：ｂｃｌ２ 蛋白的过度表达可能是端粒酶激活的重要途径之一，ｂｃｌ２ 蛋白过度表达可能通过激活端粒酶使肝细胞恶性转化导致 Ｈ Ｃ Ｃ 发生，而ｐ５３ 基因突变可能对端粒酶的激活无直接影响。Purpose To investigate relationship among bcl 2 and p53 protein expressions and telomerase activity in hepatocellular carcinomas and their surrounding liver tissues, and to study hepatocarcinogenesis. Methods In situ telomerase activity labeling was used to detect telomerase activity, and S P immunochemistry to detect bcl 2 and p53 proteins. Results The positivity rate of telomerase in HCC was higher than that in pericarcinomatous liver tissues ( P< 0 05); positivity intensity of telomerase was not related to differentiated degree of cancer cells ( P> 0 05). The positive rates of bcl 2 and p53 proteins in HCC were higher than that in pericarcinomatous liver tissues ( P< 0 05). Telomerase activity was increased with strong expression of bcl 2 protein and there was positive relation between them. Telomerase activity was not associated with expressive intensity of p53 protein in HCC and their surrounding liver tissues. Conclusions The results further support the view that overexpression of bcl 2 protein may be one of important pathways by which telomerase is activated, and speculate upon that the telomerase activation by bcl 2 overexpression may result in hepatocytes malignant transformation, and p53 mutation may unaffect telomerase activity during hepatocarcinogenesis.

关 键 词：端粒酶 肝细胞癌 BCL-2蛋白 P53蛋白 

分 类 号：R735.702[医药卫生—肿瘤]