翼状胬肉中增殖细胞核抗原的免疫组化研究  

The PCNA immunohistochemical study of pterygium

作　　者：龙心光[1] 王映芬[1] 黄应桂[2] 文立 陈毓东[1] 

机构地区：[1]广东医学院附属医院眼科,湛江524001 [2]广东医学院附属医院

出　　处：《中华眼科杂志》1999年第5期380-382,I020,共4页Chinese Journal of Ophthalmology

摘　　要：目的　观察翼状胬肉的增殖状况及用药后的变化。方法　将５４ 例翼状胬肉术前患者随机分为３ 组：Ⅰ组１８ 例为未用药组，Ⅱ组１６ 例术前局部应用氟美松，Ⅲ组２０ 例术前局部应用高三尖杉酯碱加氟美松。采用免疫组化方法对手术切除的翼状胬肉标本进行增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌｎｕｃｌｅａｒ ａｎｔｉｇｅｎ ，ＰＣＮＡ） 检测。结果　未用药组（ Ⅰ组）ＰＣＮＡ 阳性率为（１１５３ ±３６２）％ ，氟美松组（ Ⅱ组） 为（１０２８ ±４１８）％ （ Ｐ＞ ００５） ，高三尖杉酯碱加氟美松组（ Ⅲ组） 为（５５９±３８２） ％ （ Ｐ＜ ００１） 。＜ ６０ 岁的进行型患者ＰＣＮＡ阳性率高于≥６０ 岁的静止型患者。结论　翼状胬肉组织中存在细胞增殖及ＰＣＮＡ表达，术前应用高三尖杉酯碱加氟美松可抑制ＰＣＮＡ 表达及术后翼状胬肉的复发。Objective To study the proliferating status of pterygium and the drug′s influence on it. Method Fifty four patients with pterygium were randomly assigned into three groups: Before the surgical excision of the pterygium, group 1 (18 cases) had received no drug, group 2 (16 cases) received local dexamethasone treatment, and group 3 (20 cases), local homoharringtonine and dexamethasone treatment. The immunohistochemical study on proliferating cell nuclear antigen (PCNA) was performed on the surgical specimens of the pterygium. Result The PCNA positive rate was (11.53±3.62)% in the untreated group, (10.28± 4.18)% in the dexamethasone group ( P >0.05), and (5.59±3.82)% in the homoharringtonine and dexamethasone group ( P <0.01). The PCNA positive rate was higher in the younger patients (<60 years) with progressive type of the disease than that in the elder patients (≥60 years) with quiet type. Conclusions There are cell proliferation and PCNA expression in pterygium. The homoharringtonine and dexamethasone local injection before the excision of pterygium can effectively decrease the PCNA expression and recurrence rate of pterygium. Local injection of dexamethasone before surgical excision has no influence on the PCNA expression of pterygium.

关 键 词：翼状胬肉 增殖细胞核抗原 免疫组织化学 

分 类 号：R777.330.2[医药卫生—眼科]