宫颈癌HeLa细胞抑癌蛋白FBW7调控机制研究  

Study on the regulatory mechanism of tumor suppressor protein FBW7 in cervical cancer HeLa cells

作　　者：孙迪[1] 沈宜[1] 谢莹珊[1] 隆霜[1] 

机构地区：[1]重庆医科大学基础医学院干细胞与组织工程研究室,重庆400016

出　　处：《重庆医科大学学报》2011年第5期520-523,共4页Journal of Chongqing Medical University

基　　金：重庆市渝中区科委资助项目(编号:2009)

摘　　要：目的:观察MAPK信号通路阻断剂UO-126对人类宫颈癌细胞株HeLa细胞周期素E(cyclinE)及F-盒和含蛋白7的WD重复结构域FBW7表达的影响。方法:采用RT-PCR、Western blot检测UO-126阻断MAPK信号通路前后cyclin E和FBW7 mRNA及蛋白表达情况。结果:RT-PCR及Western blot结果显示,UO-126作用后HeLa细胞cyclin E mRNA表达水平无明显变化,cyclin E蛋白表达水平明显下降,FBW7 mRNA及蛋白表达水平较未处理HeLa细胞均明显增高。结论:宫颈癌HeLa细胞中FBW7异常表达与MAPK信号通路激活密切相关。UO-126阻断MAPK信号通路可下调cyclin E表达,上调FBW7表达。Objective：To investigate the effects of UO-126,a MAPK inhibitor,on the expression of cyclin E and F-box and WD repeat domain-containing protein 7（FBW7）of human cervical cancer cell line（HeLa cells）.Methods：The mRNA and protein expression of cyclin E and FBW7 were detected by RT-PCR and Western blot before and after MAPK signal was blocked by UO-126,a MAPK inhibitor.Results：RT-PCR and Western blot exhibited that cyclin E mRNA expression had no significant changes and cyclin E protein had significantly decreased after HeLa cells were treated with UO-126,and FBW7 mRNA and protein expression had significantly increased after HeLa cells were treated with UO-126.Conclusion：The abnormal expression of FBW7 is closely related to the activation of MAPK signaling pathway in cervical cancer HeLa cells.Cyclin E is decreased and FBW7 is increased after UO-126 blocks the MAPK signaling pathway.

关 键 词：UO-126 MAPK 宫颈癌细胞株(HeLa细胞) 细胞周期素E FBW7 

分 类 号：R730.23[医药卫生—肿瘤]