不同利爪小鼠对戊巴比妥钠药物反应的初步研究  

Relations between paw preferences and drug effects to Pentobarbital in mice

作　　者：薛翔[1] 丁冉[1] 曾嵘[1] 

机构地区：[1]南方医科大学基础医学院基础实验教学中心,广州510515

出　　处：《重庆医科大学学报》2011年第5期569-571,共3页Journal of Chongqing Medical University

摘　　要：目的:研究小鼠是否与人类一样存在着利手(爪)偏侧性现象,比较不同利爪偏侧性小鼠对不同浓度的戊巴比妥钠药物反应,初步探讨利爪分布特点及其对药物反应差异,以及这种差异对临床个性化用药的参考意义。方法:成年昆明鼠96只,雌雄各半,观察小鼠抓取食物所用左右前爪频次,了解小鼠利爪分布;观察不同利爪小鼠给予非麻醉剂量戊巴比妥钠20 mg/(kg.周)前后10 min内自主活动的次数;观察小鼠给予麻醉剂量戊巴比妥钠60 mg/(kg.周)后翻正反射消失时间及苏醒时间;观察不同利爪小鼠戊巴比妥钠LD50实验;了解不同利爪小鼠对戊巴比妥钠药物反应。结果:小鼠左右利爪比例约为2∶3,不同性别小鼠之间差异无统计学意义;给予非麻醉剂量戊巴比妥钠后,左利小鼠自主活动次数显著低于右利小鼠(P<0.05);给予麻醉剂量戊巴比妥钠后,左利小鼠苏醒时间显著短于右利小鼠(P<0.01),而翻正反射反射消失时间差异无统计学意义;左右利小鼠戊巴比妥钠LD50实验结果差异无统计学意义(P>0.05)。结论:小鼠的利爪差异可能导致生理及药物反应的差异,对该类现象的深入研究将有望为临床的个体化用药提供依据。Objective：To observe the effects of paw preferences on drug reaction to Pentobarbital sodium in mice.Methods：96 adult Kunming mice were separated into two groups（left-paw and right-paw）by skilled reaching test.Each group was treated with Pentobarbital sodium with sub-anesthetic dosages 20 mg/（kg·week）,anesthetic dosages 60 mg/（kg·week） and followed by LD50 test.Results：The ratio of left-paw and right-paw mice is 2 to 3,and there is no difference between two genders.Spontaneous activities of the left-paw mice are significantly lower than those of the right-paw with sub-anesthetic dosages.Sleeping duration of the left-paw is markedly shorter than the right-paw with anesthetic dosages.There is no difference in LD50 test of Pentobarbital sodium between the two groups.Conclusion：The paw preference may affect physiological reaction to drugs in mice.The deeper study on this phenomenon may provide the basis of clinically personalized medicine.

关 键 词：戊巴比妥钠 利爪 个体化用药 

分 类 号：R363.2[医药卫生—病理学]