单层上皮细胞角蛋白在口腔粘膜癌变过程中的表达  

Changes of Simple Epithelial Cytokeratin During Oral Carcinogenesis

作　　者：聂敏海[1] 李秉琦[2] 曾光明[2] 何志秀[2] 陈谦明[2] 周敏[2] 罗刚[2] 曾昕[2] 

机构地区：[1]泸州医学院口腔系 [2]华西医科大学口腔医学院,610041

出　　处：《华西口腔医学杂志》1999年第3期208-209,220,I010,共4页West China Journal of Stomatology

基　　金：四川省卫生厅科学研究基金! (编号 96 0 0 40 )

摘　　要：目的 :探讨单层上皮细胞角蛋白 CK18和 CK19作为口腔癌前病变标志的可能性。方法 :用 L SAB免疫组化染色方法检测 CK18和 CK19在福尔马林固定、石蜡包埋的口腔正常粘膜、上皮单纯增生、轻度上皮异常增生、中度上皮异常增生、重度上皮异常增生和口腔鳞癌组织中的分布和表达强度 ,光镜观察染色切片 ,结果用秩和检验分析。结果 :正常和异常的口腔粘膜均未检测到 CK 18;CK 19可表达于正常非角化上皮的基底层细胞、异常增生上皮的基底上层细胞及口腔鳞状细胞癌中 ,阳性细胞数与上皮的异常增生程度及鳞癌的分化程度相关。结论 :CK19表达于口腔上皮基底上层细胞可作为诊断口腔癌前病变和判断口腔鳞癌分化程度的候选辅助指标之一。Objective:To investigate whether the simple epithelial cytokeratin CK18 and CK19 can be used as a marker of oral precancerous lesions. Methods: Formalin fixed, paraffin embedded tissue sections of normal oral mucosa, epithelial hyperplasia, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia and oral squamous cell carcinomas were stained with a CK18 specific antibody and CK19 specific antibody respectively by LSAB immunohistochemical method. The stained sections were observed under light microscopy. The results were described and analyzed with Rank Sum Test. Results: CK18 was not detected in normal and abnormal oral tissue sections. But in normal nonkeratinized mucosa, CK19 was detected in the basal cell layer dispersively. In epithelial dysplasia, CK19 was detected in the suprabasal cell layer and the number of CK19 positive cell layers was correlated with the dysplasia degree of epithelia. Furthermore, CK19 was detected in oral squamous cell carcinoma, especially in the poor differentiated cancer cells. Conclusion: CK19 expression in suprabasal cell layer of oral mucosa can be used as a candidate marker for diagnosis of oral precancerous lesions and determination of the differentiation level of oral squamous cell carcinoma.

关 键 词：癌前病变 细胞角蛋白 口腔粘膜肿瘤 

分 类 号：R739.850.2[医药卫生—肿瘤]