人参皂苷Rg1联合骨髓间充质干细胞移植对痴呆大鼠学习记忆能力的影响  

Effect of Ginsenoside Rg1 on the Spatial Learning-memory Ability in Dementia Rats after Transplanted with Bone Marrow Mesenchymal Stem Cells

作　　者：邬伟[1] 杨景全[2] 何志勇[1] 王小同[1] 

机构地区：[1]温州医学院附属第二医院脑科康复中心,浙江325000 [2]温州医学院附属第二医院手外科,浙江325000

出　　处：《中国中西医结合杂志》2011年第6期799-802,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省卫生厅课题资助项目(No.2006B103)

摘　　要：目的探讨人参皂苷Rg1对骨髓间充质干细胞(mesenchymal stem cell,BMSCs)移植后阿尔茨海默病(Alzheimer′s disease,AD)大鼠学习记忆能力的影响及机制。方法雄性SD大鼠75只,采用随机数字表法分为双侧穹窿海马伞(hipp-ocampal fimbria-farnix,FF)切断模型组(模型组),假手术组(除不切断FF以外,其他操作同模型组),人参皂苷Rg1治疗组(Rg1组:造模后2周给予腹腔注射人参皂苷Rg1,每天1次,共4周),BMSCs移植治疗组(BMSCs组:造模后2周,每只大鼠接受1×106个BMSCs治疗)。人参皂苷Rg1联合BMSCs治疗组(联合组:人参皂苷Rg1在BMSCs移植治疗同时给药)。采用Morris水迷宫检测各组动物逃避潜伏期(s),RT-PCR检测基底前脑神经生长因子(NGF)mRNA表达。结果双侧FF切断术后6周,各用药组与模型组比较,逃避潜伏期缩短,提示痴呆大鼠学习记忆能力有所改善;联合组大鼠学习记忆能力、NGF mRNA平均表达水平分别为29.95±2.03、1.13±0.15,BMSCs组分别为44.36±1.43、0.78±0.09,联合组优于BMSCs组(P<0.05)。结论人参皂苷Rg1能够加强BMSCs移植治疗AD模型大鼠的学习记忆能力,其机制可能与人参皂苷Rg1上调BMSCs植入痴呆大鼠基底前脑神经元NGF的表达有关。Objective To study the effect and mechanism of ginsenoside Rg1 on the spatial learning-memory ability in rats with Alzheimer′s disease after transplanted with bone marrow mesenchymal stem cells（BMSCs）.MethodsUsing digital randomization table method,seventy-five male SD rats were divided into the bilateral FF transection model group（as the model group： ambi-hippocampal fimbria-fornix transected）,the sham-operative control group（the SOC group： receiving the same modeling process as the model group,but without ambi-hippocampal fimbria-fornix transected）,the ginsenoside Rg1 treatment group（as the treatment group： Two weeks after modeling ginsenoside Rg1 was peritoneally injected at the dose of 5 mg/kg,once daily for four weeks in total）,the BMSCs transplanted treatment group [as the control group： Two weeks after modeling every rat received transplantation of BMSCs（10 μL,1×106 cells）],and the ginsenoside Rg1＋BMSCs treatment group（as the combination group： They received both transplantation of BMSCs and peritoneal injection of ginsenoside Rg1）.The spatial learning-memory ability of rats was detected by Morris water maze and the escape latency（s） was recorded.mRNA expression of nerve growth factor（NGF） was detected using Real-time PCR.Results Six weeks after the hippocampal fimbria-fornix（FF） transection,the escape latency of each medication group was obviously shorter than that of the model group,and the spatial learning-memory ability of dementia rats was somewhat improved.The spatial learning-memory ability of rats in the combination group was（29.95±2.03） and the mRNA expression level of NGF was（1.13±0.15）,better than those in the BMSCs group（44.36±1.43,0.78±0.09,P0.05）.Conclusions Ginsenoside Rg1 could strengthen the spatial learning-memory ability in dementia rats after transplanted with BMSCs.Its mechanism might be possibly correlated with up-regulating mRNA expression of NGF in basal forebrain after BMSCs transplantation.

关 键 词：骨髓间充质干细胞 阿尔茨海默病 人参皂苷RG1 

分 类 号：R749.16[医药卫生—神经病学与精神病学]