高迁移率族蛋白B1在子宫内膜腺癌中的表达及意义  

Expression and significance of the HMGB1 protein in endometrial adenocarcinoma

作　　者：马本红[1] 张贵宇[1] 梁静[1] 祖增艳[2] 李越[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012 [2]山东省警官总医院妇产科,济南250002

出　　处：《山东大学学报（医学版）》2011年第6期144-148,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2006C50)

摘　　要：目的探讨高迁移率族蛋白B1(high mobility group box-1,HMGB1)在子宫内膜腺癌组织及细胞系中的表达及其临床意义。方法应用免疫组织化学染色法检测45例不典型增生子宫内膜、120例子宫内膜腺癌和20例正常子宫内膜组织中HMGB1的表达,用免疫印迹法(Western blot)分析HMGB1的表达水平;另外选取高、中、低分化子宫内膜腺癌细胞系ECC-1、HEC-1-a、KLE细胞,应用免疫细胞化学染色和Western blot检测其HMGB1的表达差异。结果 HMGB1在子宫内膜癌和不典型增生组织中的阳性表达率分别为80.00%(96/120)、66.67%(30/45),明显高于正常内膜5.00%(1/20),差异有统计学意义(P<0.05);HMGB1表达阳性率与肿瘤浸润程度、转移和手术病理分期联系密切(P<0.05),而与肿瘤的分化程度无明显联系(P>0.05)。ECC-1、HEC-1-a、KLE细胞中均发现HMGB1表达,表达水平无明显差异。结论 HMGB1在子宫内膜腺癌及癌前病变中表达水平明显升高,可能在子宫内膜癌发生和发展过程中具有重要作用。Objective To investigate expression of HMGB1 （high mobility group boxl ） in human endometrial adenocarcinoma and ECC-I, HEC-I-a and KLE cells, and explore its relation with the clinical characteristic. Methods Immunohistochemical staining was used to detect expression of HMGB1 in 45 atypical hyperplasia endometria, 120 endomelrial adenocarcinomas and 20 normal endometria, and Western blot was applied to analyze expression of I-IMGB1. Expression of HMGB1 was also detected in high, moderate and low grade endometrial adenocarcinoma ceils （ECC-1, HEC-I-a and KLE） by immunohistochemistry and Western blot. Resdts Positive expression rates of HMGB1 were 80.00% （96/120） and 66.67% （30/45） in endometrial adenocarcinoma and atypical hyperplasia endometria, respectively, which were significantly higher than 5.00% （1/20） in normal endometria. The positive expression rate of HMGB1 was significantly correlated with invasion, metastasis and operative-pathological staging of human endometrial adenocarcinoma （P 〈 0. 05 ）, while it showed no significant correlation with the grade （ P 〉 0. 05 ）. Expression of HMGB1 was positive in ECC-1, HEC-I-a and KLE cells with immunohistochemistry and Western blot, while the difference in expression level was not significant. Conclusion Over-expression of HMGB1 may play an important role in carcinogenesis and development of endometrial adenocarcinoma.

关 键 词：子宫内膜肿瘤 腺癌 高迁移率族蛋白B1 免疫组织化学 免疫印迹法 

分 类 号：R737.33[医药卫生—肿瘤]