Neurogenesis of Adipose-derived Stem Cells in Hydrogel  

作　　者：谢雪微 唐洲平 陈娟 杨洁 曾文高 刘娜 刘永明 

机构地区：[1]Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology [2]Department of Neurology, Changzheng Hospital, the Second Military Medical University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2011年第2期174-177,共4页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Sciences Foundation of China (No. 30770751);the Scientific Research Foundation of Department of Hubei Province (No. JX4A03)

摘　　要：Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells （ADSCs） were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal （NB） media supplemented with B27, basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF）. Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells （ADSCs） were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal （NB） media supplemented with B27, basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF）. Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.

关 键 词：adipose-derived stem cells HYDROGEL NEUROGENESIS tissue engineering 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]