局灶性皮质发育不良皮质中NMDA受体亚基表达情况  

Expression of NMDA receptor subunits in the cortex of focal cortical dysplasia

作　　者：郑杰[1] 李文玲[1] 刘英丽[2] 杜亚丽[1] 郭韬[1] 康进生[1] 武江[1] 

机构地区：[1]河北省人民医院功能神经外科,石家庄050051 [2]河北医科大学公共卫生学院流行病教研室,石家庄050017

出　　处：《中国微侵袭神经外科杂志》2011年第6期269-272,共4页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的探讨N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体亚基NR1、NR2A、NR2B在难治性癫病人局灶性皮质发育不良(focal cortical dysplasia,FCD)皮质中的表达及意义。方法选取20例难治性颞叶癫(术后病理证实为FCD)为FCD组及10例颞叶血管畸形病人为对照组,收集两组病人术中切除的颞叶皮质,利用免疫组化和Syber Green荧光定量PCR技术检测标本中NMDA受体亚基NR1、NR2A、NR2B蛋白和mRNA的表达情况。结果对照组NR1、NR2A和NR2B蛋白表达分别为3.5、3、3,FCD组分别为5、5、5。对照组NR1、NR2A和NR2B mRNA表达分别为1.109、1.079、1.157,FCD组分别为2.176、2.324、2.348。与对照组比较,FCD组NR1、NR2A、NR2B蛋白和mRNA表达显著增加(均P<0.01)。结论 FCD皮质中NMDA受体亚基蛋白及mRNA表达升高,可能是FCD致机制之一。Objective To explore the expression and its significance of N-methyl-D-aspartate（NMDA） receptor subunits（NR1,NR2A,NR2B） in the cortex with focal cortical dysplasia（FCD）.Methods Twenty patients with intractable temporal lobe epilepsy who were diagnosed as having FCD by postoperative pathological analysis were served as FCD group and 10 patients with vascular malformation of the temporal lobe as control group.The protein and mRNA expressions of NR1,NR2A,NR2B in the temporal lobe cortex which was removed intraoperatively were detected by immunohistochemistry staining and Syber Green-fluorescent quantitative PCR.Results The protein expressions of NR1,NR2A,NR2B in the temporal lobe cortex were 3.5,3,3 in control group and 5,5,5 in FCD group respectively.The mRNA expressions of NR1,NR2A and NR2B were 1.109,1.079,1.157 in control group and 2.176,2.324,2.348 in FCD group respectively.Compared with control group,the protein and mRNA expressions of NR1,NR2A,NR2B in FCD group were increased significantly（all P0.01）.Conclusions Increase of protein and mRNA expressions of NMDA receptor subunits（NR1,NR2A,NR2B） in the cortex with FCD might be one of epileptogenic mechanisms of FCD.

关 键 词：癫 局灶性皮质发育不良 NMDA受体 免疫组织化学 荧光定量PCR 

分 类 号：R742.1[医药卫生—神经病学与精神病学]