脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响  

Lipopolysaccharide-preconditioning influences the NO secretion from cultured cerebral astrocytes and microglia in vitro

作　　者：苏芸 谢泽锋[2] 辛岗[1] 许燕璇[1] 李康生[1] 

机构地区：[1]汕头大学医学院微生物学与免疫学教研室,广东汕头515041 [2]汕头大学医院第一附属医院,广东汕头515041

出　　处：《癌变．畸变．突变》2011年第3期176-180,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金项目(81001340;30771988);广东省医学科研基金项目(B2010211);汕头市科技计划资助项目(2009-70)

摘　　要：目的:探讨脂多糖(lipopolysaccharide,LPS)预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响。方法:体外分离、纯化、培养星形胶质细胞和小胶质细胞。培养细胞分设LPS预刺激组(先用10ng/ml LPS预刺激18h后,改用1μg/ml的LPS再刺激),LPS 10ng/ml单次刺激组,LPS 1μg/ml单刺激组与对照组(不用LPS刺激)。分别于LPS刺激后6、24、48h收集细胞培养上清,用硝酸还原酶法检测NO水平。结果:星形胶质细胞和小胶质细胞,LPS预刺激组在24h后,NO水平增加,明显高于相应时间点LPS 1μg/ml单次刺激组(P均<0.05);其中,星形胶质细胞分泌NO的时间较长,可持续到48h,与相应单次刺激组比较差异具有统计学意义(P<0.05)。结论:LPS体外预刺激神经胶质细胞,可促使细胞分泌NO的水平升高。OBJECTIVE：To study the influences of lipopolysaccharide（LPS）preconditioning on NO secretion in cultured cerebral astrocytes and microglia.METHODS：Mice cerebral astrocytes and microglia were isolated and purified in vitro.Thereafter,these glial cells were divided into 4 groups：LPS-primed group,10 ng/ml LPS treated group,1μg/ml LPS treated group and non-treated group.The LPS-primed group was preconditioned with 10 ng/ml of LPS for 18 hours.After the preconditioning,the cells received the second treatment with 1μg/ml of LPS for 6 hours, 24 hours and 48 hours.Then cultured supernatants of all 4 groups were collected.NO level in the supernatants was determined by Griess method.RESULTS：Both in cultured astrocytes and microglia,NO level of the LPS-primed group was increased at 24 hours with LPS re-treatment compared with the 1μg/ml LPS-stimulated group（P0.05）.Moreover, at 48 hours with LPS re-treatment,a long-lasting and significant enhancing effect was found in astrocytes（P0.05）. CONCLUSION：LPS preconditioning could enhance NO secretion ability in cultured cerebral astrocytes and microglia.

关 键 词：星形胶质细胞 小胶质细胞 脂多糖 预刺激 一氧化氮 

分 类 号：R365[医药卫生—病理学]