TGF-β1调控成釉细胞ODAM基因表达的研究  

TGF-β1 regulates ODAM gene expression in ameloblasts

作　　者：郝建忠[1,2] 孙学玲[1] 何永云[1] 梁广智[1] 刘晓影[1] 孙岩[1] 高玉光[1,2] 

机构地区：[1]潍坊医学院附属医院口腔科,山东潍坊261053 [2]潍坊医学院口腔医学研究所,山东潍坊261053

出　　处：《牙体牙髓牙周病学杂志》2011年第6期305-308,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30973327);山东省自然科学基金(ZR2010HM076);山东省教育厅基金(J08LH65)

摘　　要：目的:探讨TGF-β1对ODAM基因表达的作用。方法:通过实时定量RT-PCR法观察TGF-β1对成釉细胞ODAM基因表达的影响;利用c-jun小RNA干扰(siRNA)技术,阻断转录因子c-jun基因表达,观察c-jun基因沉默对TGF-β1诱导ODAM基因表达的影响;利用双荧光素酶基因报告系统研究成釉细胞中TGF-β1对ODAM启动子转录活性的调控作用。结果:TGF-β1刺激成釉细胞后,ODAM基因表达显著增强;利用小RNA干扰技术使c-jun基因沉默,实时定量RT-PCR法研究显示,TGF-β1调控ODAM基因表达的作用减弱。将pGL3-ODAM转染成釉细胞后,用TGF-β1刺激成釉细胞一定时间,ODAM启动子的转录活性增强。c-jun基因沉默抑制了TGF-β1对ODAM启动子的激活作用。结论:在釉质发育过程中,TGF-β1通过转录因子c-jun调控成釉细胞ODAM基因表达。AIM： To investigate the regulatory effects of TGF-β1 on odontogenic ameloblast-asssociated protein （ODAM） gene expression in ameloblasts. METHODS ： Cultured ameloblasts were stimulated with various concentration of TGF-β1. Eight hours after stimulation, ODAM mRNA expression was determined by real time RT-PCR. Ameloblasts were transfected with c-jun siRNA for 48 hours. The transfected ameloblasts were then stimulated with 1 ng/mL TGF-β1 for 8 hours before ODAM mRNA detection. Dual luciferase analysis was used to observe the effects of TGF-β1 on the transcriptional activity of ODAM promoter. RESULTS：TGF-β1 increased ODAM gene expression in ameloblasts. Knockdown of the transcription factor c-jun by siRNA inhibited the TGF-β1-induced ODAM expression. Luciferase analysis showed that TGF-β1 enhanced ODAM promoter activity in ameloblasts and knockdown of c- jun by siRNA blocked the TGF-β1-induced ODAM promoter activity. CONCLUSION： TGF-β1 regulates ODAM gene expression via transcription factor c-jun in ameloblasts.

关 键 词：TGF—β1 c—jun ODAM(odontogenic ameloblast—asssociated protein) 

分 类 号：R780.2[医药卫生—口腔医学]