慢性牙周炎牙龈组织细胞凋亡及凋亡相关蛋白Caspase-3的表达  

Apoptosis in gingival tissues with chronic periodontitis and expression of related apoptosis protein Caspase-3

作　　者：张轩[1] 冯利[2] 余占海[1] 张菊梅[1] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000 [2]兰州大学基础医学院,甘肃兰州730000

出　　处：《牙体牙髓牙周病学杂志》2011年第6期325-328,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:研究慢性牙周炎牙龈组织中细胞凋亡的发生情况和Caspase-3蛋白的表达,探讨其在慢性牙周炎病变发生中的意义。方法:应用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、免疫组织化学方法检测21例慢性牙周炎牙龈组织和21例健康牙龈组织中的细胞凋亡指数(apoptosis index,AI)及Caspase-3蛋白的表达。结果:慢性牙周炎组牙龈组织中细胞凋亡指数明显高于正常对照组(P<0.05)。与正常组相比,慢性牙周炎组牙龈组织中细胞caspase-3表达明显增强,两组间有显著性差异(P<0.05)。结论:慢性牙周炎病人牙龈组织细胞发生凋亡,且通过激活细胞凋亡信号传导途径中的Caspase-3而导致慢性牙周炎发生。AIM： To investigate whether apoptosis exist in gingival tissues of chronic periodontitis and to examine the expression of Capase 3 in gingival tissues. METHODS ： Gingival tissues from 21 periondontitis patients and 21 periodontally healthy individuals were collected, respectively. Apototic cells were detected by TUNEL staining and caspase-3 expression were determined by immunohistochemical staining SP method. RESULTS： The apoptosis index in gingival tissues of chronic periodontitis group was significantly higher than that in the normal group （ P 〈 0.05 ）. Compared with the normal group, the expression of Caspase-3 was significantly stronger in chronic periodontitis group （P 〈 0.05）. CONCLUSIN： Cell apoptosis might play a role in the tissue destruction of chronic periodontitis.

关 键 词：慢性牙周炎 CASPASE-3 细胞凋亡 

分 类 号：R780.2[医药卫生—口腔医学]