双乙酰-肌酸-CTMAB体系共振光散射法测定尿中痕量α-萘酚  

Determination of trace α-naphthol in urine based on diacetyl-creatine-CTMAB system by resonance light scattering method

作　　者：袁康秀[1] 袁玉坤[1] 史林飞[1] 周斌[1] 王永生[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2011年第6期1106-1108,共3页Applied Chemical Industry

基　　金：湖南省大学生研究性学习和创新性实验计划项目(湘教通[2009]320号)

摘　　要：在弱碱性介质中,α-萘酚被双乙酰氧化为α-萘醌,α-萘醌与肌酸生成荧光化合物,该化合物与十六烷基三甲基溴化铵生成离子缔合物,使体系的共振光散射增强,在1.44×10-9~2.00×10-7mol/L范围内,共振光散射强度（RLS）与α-萘酚浓度呈良好线性关系,回归方程为：△I=186.9c（×10-8mol/L）＋84.89,r=0.999 1,检出限为4.31×10-10mol/L,样品的加标回收率为96.0%~104.0%,RSD=0.80%~3.42%,该方法可用于尿液中痕量萘酚的测定。A new resonance light scattering（RLS） method is used for the determination of α-naphthol in urine.In weak alkali medium,α-NAP was oxidized by diacetyl to form α-naphthoquinone,which formed a fluorescence compound with creatine.Then the fluorescence compound reactsed with CTMAB to form ionic complexes.The intensity of RLS at λmax 580 nm was in proportion of α-NAP concentration in the range of 1.44×10-9~2.00×10-7 mol/L.The linear regression equation was ΔI = 186.9c（×10-8 mol/L） ＋ 84.89 with a correlation coefficient of 0.999 1,and the limit of detection was 4.31×10-10 mol/L.The method had been applied for the determination of α-NAP in urine.The recoveries were in the range of 96.0%~104.0%,and the RSD were 0.80%~3.42%.

关 键 词：Α-萘酚 肌酸 双乙酰 十六烷基三甲基溴化铵 共振光散射 

分 类 号：O657.32[理学—分析化学]