熊猫粪便中乳酸菌的分离鉴定与降胆固醇作用研究  

Isolation and identification of lactic acid bacteria and the study of cholesterol-reducing effect from panda excrement

作　　者：吴慧昊[1] 段龙飞[2] 张园园[2] 牛锋[1] 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北农林科技大学生命科学院,陕西杨凌712100

出　　处：《黑龙江畜牧兽医》2011年第6期14-18,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：甘肃省科技厅支撑项目(2GS064-A41-002-06);甘肃省农牧厅攻关项目(GNSW-2007-09);兰州市科技局产业化项目(2008-1-170)

摘　　要：为筛选出高效降胆固醇乳酸菌,试验利用MRS培养基从熊猫粪便中分离得到7株乳酸菌,采用硫磷铁比色法对7株乳酸菌的体外降胆固醇能力进行测定,并利用经典分类法对所分离的乳酸菌菌株进行系统的细菌学鉴定。结果表明:得到1株编号为X1的乳酸菌,该菌能够耐受pH值为3.0的酸性环境和0.3%的胆盐,在对数生长期降胆固醇能力最强,胆固醇的降低率为25.3%,确定该菌株为肠球菌属病臭肠球菌。To screen out certain lactic acid bacterium（LAB） that can lower the levels of cholesterol,seven strains of LAB were obtained from the excrement of panda with MRS medium.Cholesterol removal activity of 7 strains of LAB was tested by the colorimetric method with Ferric chloride / phosphoric acid/ sulfuric acid and identified completely by the classic classification method.The results showed that a strain of LAB numbered X1 was obtained,which was tolerant to pH 3.0 and 0.3% bile salts.The strain presented a comparatively high ability of cholesterol reducing in the logarithmic growing period and its reducing rate was 25.3%,it belonged to genus Enterococcus,E.malodoratus by the identification.

关 键 词：乳酸菌 熊猫 降胆固醇 鉴定 

分 类 号：S816.79[农业科学—饲料科学]