食品中痕量铅检测的氢化物发生-原子荧光法的改进  

The Improvement of determination method of trace lead in food by hydride generation-atomic fluorophotometry

作　　者：彭俊 杨淞 朱光辉[2] 杨蕾 叶红利 

机构地区：[1]昆明市西山区疾病预防控制中心,昆明650118 [2]云南大学现代分析测试中心,昆明650091

出　　处：《中国卫生检验杂志》2011年第6期1334-1337,共4页Chinese Journal of Health Laboratory Technology

基　　金：昆明市西山区2010年科技专项项目(20100112)

摘　　要：目的:建立一种简便准确的氢化物发生-原子荧光光谱法检测食品中的痕量铅的方法。方法:采用干法消解样品,在酸性介质中,以铁氰化钾为氧化剂,2%的盐酸为载流,样品中的铅与硼氢化钠(NaBH4)或硼氢化钾(KBH4)反应生成铅的挥发性氢化物(PbH4),将氢化物导入原子化器中,检测其荧光强度。结果:铅在0.00μg/L^50.0μg/L范围内,荧光强度值与铅浓度相关系数r>0.9990,检出限为0.094μg/L,相对标准偏差0.80%~4.70%,加标回收率82.1%~104.0%。结论:应用改进后的氢化物发生-原子荧光法检测食品中痕量铅,具有操作简便、线性关系好、检出限低、重复性好、回收率高、结果准确的优点,且分析成本低廉,便于推广应用。Objective：To establish a simple and accurate determination method for trace lead in food by hydride-generation atomic fluorophotometry.Methods： Use dry method to digest samples.In acidic medium,with potassium ferricyanide as the oxidant,2% of hydrochloric acid as the current-carrying,the lead in samples and sodium borohydride（NaBH4） or borohydride potassium（KBH4） react and product lead volatile hydride（PbH4）.Put hydride into atomizer and detect the fluorescence intensity.Results： Lead range was between 0.00 μg/L^50.0 μg/L,the correnlation coefficient of fluorescence intensity value and plumbum concentration 0.9990,DL=0.0938ug/L,RSD was between 0.80%~4.70%,spike recovery was between 82.12%~101.2%.Conclusion： The results indicate that improved hydride generation-atomic fluorophotometry is applied to detect trace lead in food,with the advantages of not only simple operation,good linear relationship,low detection limit,good repeatability,high efficiency and accuracy,but low cost and easy application.

关 键 词：食品 痕量铅 氢化物发生-原子荧光法 

分 类 号：O657.31[理学—分析化学]