莫匹罗星锂盐改良MRS检测双歧杆菌的应用研究  

Application study on examination of Bifidobacterium in MUP-MRS medium

作　　者：蔡芷荷[1] 宋曼丹[2] 周蔼怡[3] 陈秋霞[2] 严纪文[2] 朱海明[2] 帅惠萍[4] 苏丽春[3] 何天文[3] 

机构地区：[1]广东省微生物研究所,广州510070 [2]广东省疾病预防控制中心,广州510300 [3]广东环凯微生物科技有限公司,广州510663 [4]中山大学公共卫生学院,广州510080

出　　处：《中国卫生检验杂志》2011年第6期1409-1412,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨应用莫匹罗星锂盐改良MRS(MUP-MRS)进行双歧杆菌计数的最佳条件。方法:使用双歧杆菌和乳酸菌各4株对不同剂量MUP-MRS进行性能测试;对5份含乳双歧杆菌的市售样品进行乳双歧杆菌计数;并观察双歧杆菌在不同培养时间的生长情况。结果:当莫匹罗星锂盐(L i-MUP)为0.5 mg^1.0 mg/100 m l时,双歧杆菌生长率在0.98~1.19间,两歧双歧杆菌不生长;L i-MUP大于2.0 mg/100 m l时,双歧杆菌均不生长。5份样品中,L i-MUP在0.5 mg^0.75 mg/100 m l时,乳双歧杆菌计数1.0×106~3.9×108CFU/m l,生长良好;非目标乳酸菌完全受抑制(植物乳杆菌除外)。L i-MUP在1.0 mg/100 m l时,乳双歧杆菌不生长。双歧杆菌培养48 h±2 h,菌落细少;培养72 h±2 h,形成易于计数的菌落。结论:用MUP-MRS琼脂进行双歧杆菌计数时,L i-MUP的添加剂量可选0.5 mg^0.75 mg/100 m l,培养时间选择72 h±2 h。Objective：To investigate the optimum culture condition of enumeration of Bifidobacterium in MUP-MRS Medium.Methods： The productivity of 4 target strains of Bifidobacterium and Lactic acid bacteria were calculated in MUP-MRS Media with various doses of Li-Mupirocin.The 5 samples have Bifidobacterium lactic.The growth feature of Bifidobacterium were observed in different incubation times.Results： The productivity of Bifidobacterium were all among 0.98~1.19 in MUP-MRS Media with 0.5 mg^1.0 mg/100 ml of Li-Mupirocin,but Bifidobacterium bifidum failed to growth.Bifidobacterium lactic grow with Li-Mupirocin ≥2.0 mg/100 ml.The enumeration of Bifidobacterium lactic in the commercial samples was 1.0×106~3.9×108 CFU/ml with 0.5 mg^0.75 mg/100 ml of Li-Mupirocin.Non-target strains could be totally inhibited except Lactobacillus plantarum.Bafidobacterium lactic failed to growth with Li-Mupirocin in 1.0 mg/100 ml.Bifidobacterium could only form the pinpoint colonies after 48 h±2 h incubation,but all the colonies were moderate sizes after 72 h±2 h incubation.Conclusion： It is suggested that the optimum Li-Mupirocin dose was 0.5 mg^0.75 mg/100 ml and the optimum incubation time was 72 h±2 h using MUP-MRS Medium for enumeration of Bifidobacterium in food.

关 键 词：双歧杆菌 乳酸菌 MUP-MRS琼脂 生长率 

分 类 号：R446.5[医药卫生—诊断学]