肺缺血再灌注损伤时TXA_2/PGI_2平衡的变化及尼美舒利对其的影响  

Effects of nimesulide in the imbalance of thromboxance/prostacyclin system during is-chemia-reperfusion lung injury in rats

作　　者：戴雍月[1] 张晓隆[1] 邱晓晓 王方岩[1] 陈锡文[2] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035 [2]温州医学院动物实验中心,浙江温州325035

出　　处：《温州医学院学报》2011年第3期239-241,共3页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y20080246)

摘　　要：目的:探讨大鼠肺缺血再灌注损伤时TXA2/PGI2的变化及尼美舒利对其的影响。方法:健康SD大鼠30只,随机分为假手术组(S组)、缺血再灌注组(I/R组)和尼美舒利组(NIM组),复制在体大鼠肺缺血再灌注损伤模型。检测血清丙二醛(MDA)含量、超氧化物歧化酶(SOD)和黄嘌呤氧化酶(XO)活力,放射免疫法测定肺组织血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)含量并计算比值;逆转录聚合酶链反应(RT-PCR)检测肺组织环氧合酶-2 mRNA(COX-2 mRNA)的表达。结果:NIM组与IR组相比,血清MDA、XO均明显降低,SOD明显升高;TXB2明显下降(均P<0.01),6-keto-PGF1α无明显差异,COX-2 mRNA表达明显减弱(P<0.01)。结论:尼美舒利可通过下调肺组织COX-2 mRNA的表达,抑制血小板释放TXA2,调控TXA2/PGI2的平衡,增强抗氧化应激而减轻肺缺血再灌注损伤。Objective： To observe protective effects of nimesulide（NIM）on lung is-chemia-reperfusion injury（LIRI）and investigate its potential mechanism.Methods： Single lung in situ ischemia-reperfusion animal model was used.Thirty SD rats were randomly divided into three groups,sham（S）group,ischemia reperfusion（I/R）group and nimesulide（NIM）group.Malondialdehyde（MDA）,superoxide dismutase（SOD）and xanthine oxidase（XO）in serum were detected.TXB2 and 6-keto-PGF1αlevels were separately detected with radioimmunoassay after reperfusion.The expression of COX-2mRNA in lung tissue determined by RT-PCR.Results： The results showed that XO and MDA increased and SOD decreased in serum in I/R group,while the same changes happened in NIM group but less severe.TXB2 levels increased significantly while 6-keto-PGF1α had no any remarkable change during lung ischemia-reperfusion compared with the S group（P 0.01）.For the NIM group protected with nimesulide compared with group I/R,Lung TXB2 levels decreased very significantly（P 0.01）,TXB2/6-keto-PGF1αdecreased very re-markably（P 0.01）.RT-PCR demonstrated that COX-2mRNA in lung tissue of NIM group was significantly lower than those of I/R group（P 0.01）.Conclusion： The lung ischemia-reperfusion injury induces the upregulation of COX-2 in lung.Nimesulide can inhibit platelet to release TXA2,regulate TXA2/PGI2 balance and inhibite cyclooxygenase-2 expression to protect lung for ischemia-reperfusion injury.

关 键 词：尼美舒利 肺 缺血再灌注损伤 环氧合酶-2 血栓素A2 前列环素 大鼠 

分 类 号：R363.2[医药卫生—病理学]