流式免疫磁珠法检测BCR-ABL融合蛋白在白血病中的意义  

Significance of detection of BCR-ABL fusion proteins with flow immunomagenetic beads in patients with leukemia

作　　者：章圣辉[1] 毕来喜[2] 韩义香[1] 吴建波[1] 高申孟[1] 叶爱芳[1] 陈迟琪[1] 

机构地区：[1]温州医学院附属第一医院内科实验室,浙江温州325000 [2]温州医学院附属第一医院血液科,浙江温州325000

出　　处：《温州医学院学报》2011年第3期242-244,248,共4页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y20080212)

摘　　要：目的:探讨流式免疫磁珠(CBA)法检测白血病患者BCR-ABL融合蛋白的方法学评价及其意义。方法:采用CBA法检测64例白血病患者和10例正常人外周血白细胞中BCR-ABL融合蛋白表达,同时与实时定量聚合酶链反应(RQ-PCR)和荧光原位杂交(FISH)法比较。结果:正常人外周血白细胞BCR-ABL融合蛋白平均荧光强度(MFI)是10.58±3.88,64例白血病患者中BCR-ABL阳性25例,仅1例B-ALL患者检测结果与RQ-PCR结果不一致,与FISH检测结果完全一致。结论:CBA法检测BCR-ABL融合蛋白方法可靠,重复性好,简单,快速,具有良好的临床应用前景。Objective： To investigate the methodology and significance of flow immunomagnetic beads detection of BCR-ABL fusion protein in patients with leukemia.Methods： a recently described and commercialized immunoassay was used using the BCR-ABL protein kit to detect quali-tatively the BCR-ABL protein in leukemic cell lysates in 64 patients with leukemia and 10 healthy donors,and these results were compared to the real-time quantitative PCR and the FISH analysis of BCR-ABL fusion gene in the same samples.Results： The mean fluorescence intensity of BCR-ABL fusion protein in peripheral white cell was 10.58±3.88 in healthy donors.The BCR-ABL positive patients were 25 in 64 patients with leukemia,which were consistent with those results detected by FISH,only one case of B-ALL was not consistent with those results detected by RQ-PCR.Conclusion： Flow immunomagnetic beads detection of BCR-ABL fusion protein is reliable,credible,reproducible,easily performed,excellent prospects for clinical application.

关 键 词：BCR-ABL融合蛋白 白血病 细胞微球阵列 流式免疫磁珠法 

分 类 号：R733.7[医药卫生—肿瘤]