酶细胞交联物在膜反应器中裂解青霉素的反应特征  

Reaction character of cocross-linked enzyme-cell hydrolyzing PGK in membrane reactor

作　　者：杨海波[1] 曲天明[2] 虞星炬[2] 苏志国[1] 

机构地区：[1]大连理工大学化工学院,大连116012 [2]中科院大连化物所,大连116023

出　　处：《膜科学与技术》1999年第6期30-33,37,共5页Membrane Science and Technology

摘　　要：将通过戊二醛交联得到的酶细胞共交联物截留于中空纤维膜反应器中，研究了催化剂负载量与膜透量和表观酶活以及比活的关系．考察了固定化酶细胞交联物膜反应器裂解青霉素钾盐（ＰＧＫ） 的反应特征和使用寿命．结果表明，在内径１５ ｍ ｍ 、有效长度１７５ ｍ ｍ 的膜反应器内装膜１５０根、膜有效表面积６６０ ｃｍ ２ 、负载５ ．０ ｇ 的酶细胞交联物时，裂解２５ 批８ ％ 的ＰＧＫ 可在９０ ｍｉｎ 内使其裂解率达到９６ ％ ．此结果显示，采用这种截留有酶细胞共交联的膜生物反应器裂解ＰＧＫ 有希望成为目前工业酶法生产６ － ＡＰＡ 的可选技术．Cocross-linked enzyme-cell was held-back in hollow fiber membrane reactor after enzyme and cells were cross-linked together with glutaraldehyde. The relations between catalyst loading and membrane flux, and its apparent enzyme activity and its apecific activity were studied. The reaction character of cocross-linked enzyme-cell hydrolyzing PGK and the expectancy of catalyst were investigated when cocross-linked enzyme-cell was held- back in membrane. The results showed that 25 batches of 8% PGK were hydrolyzed by the catalyst within 90?min, the conversion was up to 96%, when 5.0?g cocross-linked enzyme-cell was held- back in membane reactor with 15?mm inner diameter and 175?mm effective length, which contained 150 membranes and the effective surface area of membrane was 660?cm 2. The results provided this immobilized catalyst which was obtained by cross-linking enzyme and cells together for becoming competitive power with the current industrial enzymatic producing 6-APA when it was held-back in membrane reactor.

关 键 词：酶细胞交联物 膜反应器 青霉素 裂解 抗菌素 

分 类 号：TQ465.1[化学工程—制药化工] TQ028.8