RP-HPLC法测定火炭母药材中鞣花酸的质量分数  

Determination of Ellagic acid in Polygonum chinense L. by RP-HPLC

作　　者：叶青美[1] 江仁望[1,2] 田海妍[1] 姜飞[1] 张建龙[1] 叶文才[1,2] 

机构地区：[1]暨南大学中药及天然药物研究所,广东广州510632 [2]暨南大学中药药效物质基础及创新药物研究广东省高校重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2011年第3期300-303,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金团队项目(8351063201000003);教育部新世纪优秀人才计划项目(NCET-08-0612);中央高校基本科研业务费专项资金项目(21609202);广东省高层次人才项目(JN2010)

摘　　要：采用色谱柱为Welch Materials XB-C18柱(4.6 mm×250 mm,3μm),流动相为V甲醇∶V体积分数为0.2%的甲酸水溶液=45∶55,流速为0.8 mL.min-1,柱温为30℃,检测波长为357 nm.结果显示,鞣花酸对照品的峰面积与进样量在0.067～2.68μg的范围内呈良好的线性关系(r=0.999 9),该方法的平均回收率为99.6%,RSD为1.09%,测得10份样品中鞣花酸的质量分数在0.592～3.439 mg.g-1范围内.本方法快速、简便、重复性好,可用于火炭母药材中鞣花酸的含量测定.Ellagic acid in Polygonum chinense L. was determined by a RP-HPLC method using s XB- C18 column （4. 6 mm× 250 mm, 3 μm）. The mobile phase consisted of methanol and 0. 2% formic acid at a flow rate of 0. 8 mL/min. The detection wavelength was set at 357 nm and the column temperature was 30 ℃. The results show that the linear range of ellagic acid was 0. 067 - 2.68 μg （ r = 0. 9999）. The average recovery was 99.6 % （ RSD = 1.09 % ） , and the content of ellagic acid in ten samples was in the range of 0. 592 - 3.439 mg · g^- 1 This method is found to be quick, simple and reproducible, and it is quite suitable to be used for the determination of ellagic acid in Polygonum chinense L.

关 键 词：火炭母 鞣花酸 RP—HPLC法 含量测定 

分 类 号：R914.1[医药卫生—药物化学]