Effect of Different Energy Sources on Ochrobactrum anthropi Bacteria Chromium Reduction  

作　　者：马晓艳 潘晓鸿 程扬健 吴兆贤 潘丹梅 陈志 栗斌 林璋 关雄 

机构地区：[1]State Key Laboratory of Structural Chemistry,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences [2]Key Lab of Biopesticide and Chemical Biology,Fujian Agriculture and Forestry University,Ministry of Education

出　　处：《Chinese Journal of Structural Chemistry》2011年第6期819-826,共8页结构化学（英文）

基　　金：Supported by the National Basic Research Program of China (973 program,No. 2007CB815601,2010CB933501);the National Natural Science Foundation of China (40772034, 40902097); the Outstanding Youth Fund (50625205); the Opening Project of Key Laboratory of Solid Waste Treatment and Resource Recycle (No. 09zxgk05),Ministry of Education;the CAS Foundation (KJCX1.YW.07)

摘　　要：Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.

关 键 词：Ochrobactrum anthropi CTS-325 chromium-reduction energy sources 

分 类 号：X172[环境科学与工程—环境科学]