Spiegelmer NOX 1255对LHRH-PE40与HeLa细胞上LHRH受体结合的抑制作用  

Inhibitory Effect of Spiegelmer NOX 1255 on Binding of Luteinizing Hormone-releasing Hormone(LHRH)-Pseudomonas aeruginosa Exotoxin 40 to LHRH Re-ceptor on HeLa Cells

作　　者：邓欣[1] Sven Klussmann 李亘松[1] 聂志伟[1] 宋阳[1] 张国利[3] 朱平[3] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳110001 [2]德国柏林NOXXON制药公司 [3]军事医学科学院军事兽医研究所生物技术应用研究室,长春130062

出　　处：《中国生物制品学杂志》2011年第6期657-659,664,共4页Chinese Journal of Biologicals

基　　金：辽宁省教育厅基金(2008738)

摘　　要：目的探讨Spiegelmer NOX 1255对促黄体激素释放激素-绿脓杆菌外毒素40(Luteinizing hormone-releasing hor-mone-Pseudomonas aeruginosa exotoxin 40,LHRH-PE40)与HeLa细胞上促黄体激素释放激素(LHRH)受体结合的抑制作用。方法分别将25μmol/L的LHRH-PE40、PEA和PE40与HeLa细胞共孵育24 h后,观察细胞的形态学变化;用Spiegelmer NOX1255特异性结合LHRH-PE40上的LHRH,光学显微镜观察Spiegelmer NOX 1255对LHRH-PE40的抑制作用,ELISA检测Spiege-lmer NOX 1255对LHRH-PE40与LHRH受体结合的抑制作用。结果 LHRH-PE40和PEA可直接杀伤HeLa细胞,而PE40不能对HeLa细胞产生毒性作用;Spiegelmer NOX 1255能够结合LHRH-PE40中的LHRH,抑制LHRH-PE40与LHRH受体的结合及PE40的毒性作用。结论 LHRH-PE40对HeLa细胞的毒性作用是通过细胞表面的LHRH受体产生的;Spiegelmer NOX 1255可阻断LHRH-PE40与HeLa细胞表面LHRH受体的结合。Objective To investigate the inhibitory effect of Spiegelmer NOX 1255 on the binding of luteinizing hormone-releasing hormone（LHRH）-Pseudomonas aeruginosa exotoxin（PE） 40 to LHRH receptor on HeLa cells.Methods HeLa cells were co-incubated with 25 μmol/L LHRH-PE40,PEA and PE40 for 24 h respectively and observed for morphological change.The inhibitory effect of Spiegelmer NOX 1255 on LHRH-PE40 was observed by optical microscopy,while that on binding of LHRH-PE40 to LHRH receptor by ELISA.Results LHRH-PE40 and PEA killed HeLa cells directly,while PE40 showed no toxic effect on HeLa cells.Spiegelmer NOX 1255 could bind to the LHRH in LHRH-PE40 and inhibit the binding of LHRH-PE40 to LHRH receptor as well as the toxic effect of PE40.Conclusion The toxic effect of LHRH-PE40 on HeLa cells was associated with LHRH receptor.Spiegelmer NOX 1255 may block the binding of LHRH-PE40 to LHRH receptor on the surface of HeLa cells.

关 键 词：受体 LHRH HELA细胞 Spiegelmer NOX 1255 LHRH-PE40 

分 类 号：R737.33[医药卫生—肿瘤]