miRNA-29c抑制前列腺癌细胞系LNCaP的增殖和侵袭  

Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP

作　　者：邓震[1] 唐亮[1] 矫力[1] 许传亮[1] 张振声[1] 曽钦松 孙颖浩[1] 

机构地区：[1]第二军医大学长海医院泌尿外科,上海200433

出　　处：《第二军医大学学报》2011年第6期607-611,共5页Academic Journal of Second Military Medical University

摘　　要：目的观察miRNA-29c(miR-29c)对前列腺癌细胞系LNCaP增殖和侵袭能力的影响,探讨其潜在的应用价值。方法采用qRT-PCR观察雄激素依赖性(LNCaP)与雄激素非依赖性(LNCaP-AI)前列腺癌细胞中miR-29c的表达差异;采用miR-29c抑制物(anti-miR-29c)下调LNCaP细胞中miR-29c的表达量,绘制LNCaP细胞生长曲线,利用流式细胞仪测定细胞周期;Transwell侵袭实验分析miR-29c抑制前后LNCaP细胞的体外侵袭能力。结果 qRT-PCR显示LNCaP-AI细胞中miR-29c水平明显低于LNCaP细胞(P<0.05);下调LNCaP细胞中miR-29c的表达量,可明显促进LNCaP细胞体外增殖及侵袭能力(P<0.05)。结论 miR-29c的正常表达有利于抑制LNCaP细胞的体外增殖和侵袭,有望成为前列腺癌生物治疗的潜在靶点。Objective To observe the effect of microRNA 29c on the proliferation and invasion abilities of human prostate cancer cell line LNCaP, and to discuss its potential application. Methods The difference in miR-29c expression between ADPC （androgen-dependent prostate cancer） and AIPC （androgen independent prostate cancer） cell lines was observed by real time RT-PCR. MiR-29eqnhibitor（anti rrliR-29e）was used to decrease miR-29c expression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP eells were observed by transwell invasion assay before and after treatment with anti-miR-29c. Results The result of real-time RT PCR showed that miR-29e expression in LNCaP AI cells was significantly lower than that in LNCaP cells （P〈0.05）. Downexpression of miR-29e in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells （P（0.05）. Conclusion Normal expression of miR 29e plays an inhibitory role in the proliferation and invasion of LNCaP cells, indicating it might be a new target for biotherapy of prostate cancer.

关 键 词：前列腺肿瘤 miR-29c 细胞增殖 肿瘤浸润 

分 类 号：R737.25[医药卫生—肿瘤]