茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制  

Anti-obesity effects and its mechanism of tea polyphenols on obesity rats induced by olanzapine

作　　者：钟满群 唐言利[2] 吴铎[2] 吕俊华[2] 

机构地区：[1]白云区人民医院,广东广州510500 [2]暨南大学药学院,广东广州510632

出　　处：《中国新药与临床杂志》2011年第6期449-452,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：广州市白云区科技计划项目(2009-sz-13)

摘　　要：目的探讨茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制。方法 SD雌性大鼠50只,随机分为正常对照组(10只)和奥氮平诱导肥胖模型组(40只)。肥胖模型组于黑暗时相前1h奥氮平1.2 mg·kg^(-1)·d^(-1)灌胃,正常对照组给予等容量蒸馏水,连续2 wk。于wk 3开始,再将肥胖模型大鼠随机分为4组:模型组、西布曲明(2.5 mg·kg^(-1)·d^(-1))组、茶多酚低(150 mg·kg^(-1)·d^(-1))剂量组和茶多酚高(300 mg·kg^(-1)·d^(-1))剂量组,每组10只。连续给药5 wk。观察大鼠体重、肾和子宫周围脂肪湿重,计算Lee's指数和脂肪系数,测定血清瘦素、脂联素水平以及超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与正常对照组相比,模型组大鼠体重和脂肪湿重及Lee's指数和脂肪系数均增加,血清瘦素水平升高,脂联素水平降低(P<0.05)。与模型组比较,茶多酚低、高剂量组大鼠体重和脂肪湿重降低,Lee's指数、脂肪系数降低,SOD活性增加,MDA含量减少(P<0.05)。与模型组比较,茶多酚高剂量组血清脂联素水平升高(P<0.05),瘦素水平无显著差异(P>0.05)。结论茶多酚对奥氮平诱导的肥胖大鼠有减肥作用,其作用机制可能与增强脂联素的调节和提高抗氧化能力有关。AIM To study the anti-obesity effects of tea polyphenols （TP） on olanzapine induced weight gain in rats and its mechanism. METHODS Fifty female SD rats were randomized into 2 groups, 10 rats in the normal control group and 40 rats in the obesity model group. The obesity model rats received olanzapine （1.2 mg·kg^-1·d^-1） daily via gavage 1 h before the beginning of the dark-phase for 2 weeks, while the rats in the normal control group received an equivalent volume of distilled water. At the beginning of wk 3, the obesity model rats were randomized into 4 groups： model group （distilled water）, sibutramine （2.5 mg·kg^-1·d^-1）group, low-dose （150 mg·kg^-1·d^-1） group and high-dose （300 mg·kg^-1·d^-1） group of TP, 10 rats in each group, and the rats were administered with olanzapine continuously. TP and sibutramine were given by garage for 5 weeks. The body weight and fat weight around the kidney and uterus were measured, and Lee＇ s index and fat coefficient were calculated. The levels of serum leptin and adiponectin （ADP） , superoxide dismutase （SOD） activity and malondialchehyche （MDA） content were tested respectively. RESULTS Compared with the normal control group, the body weight, fat weight, Lee＇ s index, fat coefficient, the level of serum leptin were significantly increased and serum ADP decreased significantly in the model group （P 〈 0.05）. Compared with the model group, the body weight, fat weight, Lee＇ s index, fat coefficient and the content of MDA decreased significantly, while the SOD activity increased significantly in the low-dose group and high-dose group of TP （P 〈 0.05）. Compared with the model grouP, the level of serum ADP increased significantly in the high-dose group （P 〈 0.05） , but there was no obvious difference in the level of leptin （P 〉 0.05） . CONCLUSION TP can reduce olanzapine induced weight gain in rats, which may be associated with the enhanced regulation of ADP and antioxidative ability.

关 键 词：肥胖症 瘦素 脂联素 茶多酚 奥氮平 

分 类 号：R977[医药卫生—药品]