HSP70在兔SAH后基底动脉中的表达及其意义  

Expression of HSP 70 in the basilar arteries of rabbits after experimental subarachnoid hemorrhage and its meanings

作　　者：李仙锋[1] 叶伟[1] 王晓峰[1] 孙颖[1] 杨东波[1] 杨海城[1] 蒋传路[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经外科,哈尔滨150086

出　　处：《中国临床神经外科杂志》2011年第6期350-352,共3页Chinese Journal of Clinical Neurosurgery

基　　金：黑龙江省自然科学基金项目(NO:D2005-12)

摘　　要：目的探讨兔蛛网膜下腔出血(SAH)后基底动脉中热休克蛋白70(HSP70)的表达及其意义。方法将15只成年日本大耳白兔随机分成对照组(3只)和SAH组(12只)。采用枕大池一次注血法建立SAH模型,建模后第3、7、14和21天观察基底动脉大体和组织学变化,应用免疫组织化学染色测定基底动脉血管内皮细胞HSP70的表达。结果 SAH后第3、7和14天HSP70在基底动脉内皮细胞表达明显减少(P<0.01),第21天恢复正常。结论 SAH后基底动脉中HSP70表达水平的动态变化提示SAH后脑血管痉挛可能与血管中HSP70表达水平下降有关。Objective To study the role of heat shock protein 70 （HSP70） in the cerebral vasospasm after subarachnoid hemorrhage （SAH） in rabbits.Methods Fifteen Japanese rabbits were randomly divided into control group （n=3） and experimental groups （n=12）,who were redivided into 4 subgroups of 3 animal each according to the time from SAH to sacrifice.The SAH was produced in the rabbits of all the experimental groups by injecting autologous fresh arterial blood into the cisternae magna.The basilar arteries were taken from the animals,who in the experimental group were sacrificed respectively 3,7,14 and 21 days after SAH.The gross and histological changes in the basilar arteries of all the rabbits were observed.The expressions of HSP70 in the basilar arteries of all the rabbits was determined by immunohistochemical technique.Results The gross and histological changes in the basilar arteries after SAH included thickening of the arterial walls,narrowing of the arterial lumina,swelling and deformation of the endothelial cells,wrinkling of the elastic interna and so on.The expression levels of HSP70 in the endothelial cells of the basilar arteries were significantly lower 3,7 and 14 days after SAH than that in the control group （P 0.01）.Conclusion It is suggested that the cerebral vasospasm after SAH may be related to the decrease in HSP70 expression level in the cerebrovascular endothelial cells.

关 键 词：热休克蛋白70 脑血管痉挛 蛛网膜下腔出血 

分 类 号：R743.35[医药卫生—神经病学与精神病学] Q786[医药卫生—临床医学]