Ig基因重排检测在B细胞性淋巴瘤诊断中的应用  

Analysis of Ig gene rearrangements for diagnosis of B-cell lymphomas

作　　者：陈玥[1] 王丽[1] 潘鑫艳[1] 崔静[1] 蔡琳[1] 黎贵芸[1] 杨举伦[1] 

机构地区：[1]成都军区昆明总医院病理科,昆明650032

出　　处：《诊断病理学杂志》2011年第3期186-189,共4页Chinese Journal of Diagnostic Pathology

摘　　要：目的探讨Ig基因重排检测在B细胞性淋巴瘤中的诊断价值。方法选取B细胞性淋巴瘤30例、淋巴组织反应性增生30例,提取DNA,应用BIOMED-2引物系统中的47条引物进行PCR扩增,核酸分子异源双链凝胶电泳分析Ig基因重排结果。结果 30例B细胞性淋巴瘤中检测出Ig克隆性重排,包括IGH(A+B)克隆性重排23例,IGK克隆性重排23例,IGL克隆性重排5例,IGH(A+B)+IGK克隆性重排30例,IGHA+IGK克隆性重排29例;在30例淋巴组织反应性增生中未检测到Ig克隆性重排。结论 Ig基因重排是诊断B细胞性淋巴瘤的有用工具。Objective To discuss the Ig gene rearrangements in the diagnosis of B-cell lymphomas.Methods Formalin-fixed and paraffin-embedded samples including 30 cases of B-cell lymphomas and 30 cases of reactive lymphoid hyperplasia were chosen for extracting genomic DNA and PCR amplification using 47 BIOMED-2 primers.PCR products were analyzed by heteroduplex and polyacrylamide gel electrophoresis.Results Ig gene rearrangements were found in all 30 cases of B-cell lymphomas,including 23 cases of IGH（A＋B） gene monoclonal rearrangements,23 cases of IGK gene monoclonal rearrangements and 5 cases of IGL gene monoclonal rearrangements.30 cases（100%） of IGH（A＋B） ＋ IGK gene rearrangements were detected,29 cases（96.8%） of IGHA ＋ IGK gene rearrangements were detected,but no clonal Ig gene rearrangements were found in 30 cases of reactive lymphoid hyperplasia.Conclusions The detection of Ig gene rearrangements can be applied in the diagnosis of B-cell lymphomas at early stage.

关 键 词：基因重排 非霍奇金淋巴瘤 B细胞性淋巴瘤 BIOMED-2 

分 类 号：R733.1[医药卫生—肿瘤]