前列腺干细胞抗原的表达在乳腺浸润性微乳头状癌中的意义  

PSCA expression in invasive micropapillary carcinoma of breast

作　　者：郝金燕[1] 杨壹羚[1] 李帅[1] 钱晓龙[1] 刘芳芳[1] 付丽[1] 

机构地区：[1]天津医科大学附属肿瘤医院乳腺病理研究室教育部乳腺癌防治重点实验室天津市肿瘤防治重点实验室,300060

出　　处：《中华病理学杂志》2011年第6期382-386,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金重点项目（30930038）;教育部长江学者乳腺癌创新团队奖励计划（IRT0743）;国家863重大项目（2006AA02A249）;国家973重大项目（2009CB521700）;天津市应用基础与前沿技术计划项目（08JCZDJC23700）

摘　　要：目的探讨乳腺浸润性微乳头状癌（IMPC）中前列腺干细胞抗原（PSCA）在蛋白和mRNA水平的表达及其与临床病理特征的关系。方法采用免疫组织化学（LSAB法）检测66例IMPC、67例非特殊型浸润性导管癌（IDC-NOS）中PSCA的表达，比较其差异并分析PSCA的表达与IMPC临床病理特征之间的关系。采用逆转录聚合酶链反应（RT-PCR）检测10例IMPC和10例IDC-NOS癌组织及其对应的正常乳腺组织中PSCA mRNA的表达水平。结果免疫组织化学染色结果显示，PSCA在66例IMPC中有47例高表达（71．2％，47／66），67例IDC-NOS中35例高表达（52．2％，35／67），两组表达水平之间的差异具有统计学意义（P=0．024）；IMPC中PSCA的表达与淋巴结转移呈正相关（P=0．039）。RT-PCR结果显示，PSCA在10例IMPC和10例IDC-NOS癌组织中各有7例和5例表达，且全部高于对应的正常乳腺组织；IMPC癌组织中PSCA表达强度明显高于IDC-NOS癌组织。结论PSCA的表达可能在IMPC淋巴结转移过程中发挥了重要作用。Objective To study the expression of prostate stem cell antigen （PSCA） at protein and mRNA levels in invasive mieropapillary carcinoma of the breast （IMPC） and to analyze the relationship between PSCA expression and clinieopathologic features. Methods The expression of PSCA protein was analyzed by immunohistoehemistry （LSAB） in 66 cases of IMPC and 67 cases of invasive ductal carcinoma, not otherwise specified （IDC-NOS）. The association between PSCA expression and clinicopathologic features was also analyzed in IMPC. Furthermore, RT-PCR was used to detect PSCA mRNA in 10 cases of primary IMPC and 10 cases of primary IDC-NOS with paired normal breast tissues, each from the same subject. Results Immunohistochemieal analysis revealed the overexpression of PSCA in 47 of 66 （71.2%） cases of IMPC and 35 of 67 （52.2%） IDC-NOS. Statistical analysis showed a significant difference of PSCA expression between IMPC and IDC-NOS （ P = 0. 024）. In IMPC, the expression of PSCA was correlated with lymph nodes metastasis （ P = 0. 039）. RT-PCR showed the mRNA level of PSCA was significantly higher in primary IMPC and IDC-NOS tissue than that in paired normal breast tissue （7/10 and 5/10, respectively）, and it was also significantly higher in primary IMPC tissue than that in IDC-NOS tissue. Conclusion PSCA might play an important role in lymph node metastasis in IMPC.

关 键 词：乳腺肿瘤 癌 乳头状 肿瘤转移 前列腺干细胞抗原 

分 类 号：R737.9[医药卫生—肿瘤]