多克隆抗胸腺细胞球蛋白对白血病细胞增殖抑制及凋亡诱导作用  

Effect of polyclonal rabbit anti-thymocyte globulin on proliferation and apoptosis of human leukemia cells

作　　者：刘慧霞[1] 王椿[1] 秦尤文[1] 王小蕊[1] 杨一宁[1] 蔡宇[1] 赵初娴[1] 朱骏[1] 

机构地区：[1]上海交通大学附属第一人民医院血液科,上海200080

出　　处：《内科理论与实践》2011年第3期208-213,共6页Journal of Internal Medicine Concepts & Practice

基　　金：2009年度院企合作青年科技种子基金(项目编号:院企0910)

摘　　要：目的:探讨多克隆兔抗人胸腺细胞球蛋白(ATG)对白血病细胞的生长抑制以及诱导凋亡作用。方法:体外培养Jurkat、Raji、HL-60、NB4、K562、U937细胞株以及15例患者原代白血病细胞。用50、100、150、200、250μg/mL终浓度的ATG在不同时间分别作用于以上细胞,采用细胞计数试剂盒(CCK-8)比色法检测细胞增殖抑制率,应用流式细胞术测定细胞凋亡率。结果:ATG对Jurkat、Raji、HL-60细胞有明显的增殖抑制以及诱导凋亡作用,且高浓度ATG抑制作用更强。ATG对NB4、K562、U937细胞增殖抑制与诱导凋亡作用均不明显。15例初发白血病患者中7例的原代细胞经ATG作用后明显凋亡,凋亡率均>30%。结论:ATG具有广谱抗白血病作用,尤其是对淋巴细胞白血病作用较强,为其在异基因造血干细胞移植中的应用提供了实验依据。Objective To study the effect of polyclonal rabbit anti-thymocyte globulin（ATG） on the proliferation and apoptosis of human leukemia cells.Methods Cells including Jurkat,Raji,HL-60,NB4,K562,U937 cell lines and primary leukemia cells of 15 leukemia patients were cultured in vitro.Cells were treated with ATG at a dose from 50 to 250 μg/mL（50,100,150,200,250 μg/mL） for different times.The effect of ATG on proliferation were determined by cell counting kit-8（CCK-8） assay,and flow cytometry was used to assay the percentage of apoptosis.Results ATG could inhibit the growth of Jurkat,Raji,HL-60 cells and induce apoptosis in a does-dependent manner.However,no obvious effects of ATG on proliferation and apoptosis of NB4,K562,U937 cells were observed.Induction of apoptosis was observed in primary cells of 7 of the 15 leukemia patients treated with 250 μg/mL of ATG,and the apoptosis rates were more than 30%.Conclusions Polyclonal ATG had a broad-spectrum anti-leukemia effect,especially for lymphocytic leukemia cells.This study provides an experimental evidence for the application of ATG in allogeneic hematopoietic stem cell transplantation.

关 键 词：白血病 多克隆抗胸腺细胞球蛋白 增殖抑制 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]