镉免疫毒性机理的初步探讨  

Study of the mechanism on immunotoxicity of cadmium

作　　者：张铣[1] 薛彬[1] 魏雪涛[1] 雷志明[1] 刘湛[1] 周弘[1] 

机构地区：[1]北京医科大学卫生毒理学教研室,北京100083

出　　处：《中国环境科学》1999年第6期530-535,共6页China Environmental Science

基　　金：卫生部基金!94-1-268

摘　　要：从免疫细胞功能调控因素初步探讨镉免疫毒性机理０．３－２．４ｍｇ／ｋｇ／ｄ氯化镉连续给小鼠灌胃１４ｄ，观察免疫器官重量及免疫功能、淋巴细胞内钙离子浓度、钙调素（ＣａＭ）含量、巨噬细胞内ＮＯ、Ｏ２－、Ｈ２Ｏ２变化。结果是０．３－２．４ｍｇ／ｋｇ／ｄ氯化镉对免疫器官重量未见有明显影响，但０．３ｍｇ／ｋｇ／ｄ组腹腔灌洗液巨噬细胞总数减少、体液免疫和巨噬细胞吞噬功能抑制，淋巴细胞胞内钙离于增加、钙调素呈下降趋势，巨噬细胞ＮＯ生成、Ｏ２－减少、Ｈ２Ｏ２有减少趋势体外试验５０μｍｏｌ／Ｌ氯化镉出现抑制淋巴细胞转化、ＩＬ－２减少、胞内钙离子增加。In order to study the mechanism on the immunotoxicity of cadmium initially from the view of the regulationand control of the function of immunocyte, mice were treated with 0.3-2.4mg/kg/d CdCl2 by gavage for 14 days. After thetreatment of face the changes of weight of immune organs, immune function, the intercellular concentration of Ca2+ inlymphocyte, the amount of calmodulin in lymphocyte, the NO, O2-,H2O2 in macrophage were tested. The results didn'tshow any change on the weight of immune organs after 0.3-2.4mg/kg/d CdCl2 treatment; but the total number of cells inperitoneal lavage from the face treated with 0.3mg/kg/d CdCl2 showed decrease. There appeared inhibition in the function of humoral immunity and phagocytic function of macrophage, the increase on the concentration of [Ca2+], when treated with 0.3mg/kg/d CdCl2. The production of NO, O-2 in macrophage also decreased at this dosage. In vitro study,50umol/L CdCl2 could induce the inhibition of lymphocyte proliferation, the decrease of IL-2, the increase of [Ca2+]concentration and the content of CaM also had the tendency to decrease. So we thought that the immunotoxicity of cadmium might have close relation with the imbalance of Ca2+ stability in lymphocyte induced by cadrium.

关 键 词：镉 免疫毒性 钙稳态失衡 污染物质 

分 类 号：X132[环境科学与工程—环境科学]