钛表面形貌对人单核细胞活力和粘附影响的实验研究  

Influence of Surface Microstructure of Titanium on Viability and Adhesion of Human Monocytes

作　　者：邓天燕[1] 王少安[2] 谢佐理 龙文[1] 

机构地区：[1]江门市口腔医院种植科,广东江门529000 [2]四川大学华西口腔医学院

出　　处：《广东牙病防治》2011年第6期283-288,共6页Journal of Dental Prevention and Treatment

摘　　要：目的研究钛金属表面形貌对人单核细胞活力和粘附的影响。方法应用机械磨光、酸蚀、喷砂、喷砂酸蚀的表面处理方式形成4种不同的钛片表面形貌,分别设为SiC组、SiC+F组、63号组和63+F组。表面粗糙度仪检测钛试件表面粗糙度,以Ra值表征,扫描电镜观测钛试件表面形貌。将离心分离的健康成年人外周血单核细胞接种于处理后钛试件表面,扫描电镜观察培养48 h钛片表面单核细胞粘附情况;四唑盐比色法检测和钛片共同培养1、3、5、7 d的细胞活力。结果 4种钛片表面单核细胞的活力和粘附有差异,置于Ra值最大的63号组培养的单核细胞在第1天、第3天、第5天、第7天都显示了最高的活力,置于Ra值相近的SiC+F组和63+F组培养的细胞显示了相似的活力。扫描电镜下观察,表面粗糙的63号组钛片表面粘附了较多的单核细胞,表面多微孔的63+F组有大量的单核细胞粘附在材料表面的窝洞结构中。结论钛表面形貌影响人单核细胞的活力和粘附。表面粗糙度大促进单核细胞的活力,表面粗糙度大和表面多微孔结构有利于单核细胞的粘附。Objective The purpose of the study was to investigate the effects of surface topography of titanium on viability,adhesion of human monocytes,so as to provide more information of fundamental research concerning the interaction of titanium surface topography and bioactivity of human monocytes.Methods Treat the pure titanium plates with SiC paper-grinding,acid-etching,sandblasting,sandblasting and large-grits acid-etching（SLA）,labeled as SiC group,SiC＋F group,No.63 group and 63＋F group,respectively.Surface topography was observed by scanning electron microscope（SEM） and parameter Ra was used to describe the surface roughness.3-（4,5-dimethy1-2-thiazolyl）-2,5-diphenyl-2H-tetrazoliumbromide（MTT） assay was used to assess the viability and SEM was given to evaluate the adhesion of human monocytes co-cultured with titanium plates.Results Viability and adhesion of human monocytes was different on different surface topography of titanium plates.Viability of monocytes of No.63 group was the highest on the 1st day,the 3rd day,the 5th day,and the 7th day.Monocytes of SiC＋F group and 63＋F group had similar viability.More monocytes adhere to the surfaces of 63＋F group and No.63 group.Conclusion Surface topography modulates viability and adhesion of human monocytes in vitro.Surface roughness may enhance viability of monocytes.Increased surface roughness and porous micro-structure favor adhesion of monocytes.

关 键 词：钛 表面形貌 单核细胞 细胞活力 细胞粘附 

分 类 号：R783.1[医药卫生—口腔医学]