大鼠舌鳞癌诱变过程中基质金属蛋白酶13表达变化的研究  

Expression of Transcription Level of Matrix Metalloproteinases-13 in a Rat Model of Tongue Squamous Cell Carcinoma

作　　者：陈中利[1] 赵建江[1] 王治平[1] 韩久松[1] 邱小玲[1] 

机构地区：[1]南方医科大学附属口腔医院.广东省口腔医院,广东广州510280

出　　处：《广东牙病防治》2011年第6期309-312,共4页Journal of Dental Prevention and Treatment

基　　金：广东省科技计划项目(2009B060700058);广东省科技计划项目(2008B030301183);广东省科技计划项目(2010A36040836)

摘　　要：目的探讨基质金属蛋白酶13(matrix metalloproteinases 13,MMP-13)在大鼠舌鳞癌诱变过程中转录水平表达变化及其临床意义。方法 50只SD大鼠随机抽取10只作为正常对照组,给予正常饮食。另40只每天喂养0.002%4-硝基喹啉-1-氧化物,于16周时处死20只,选取处于上皮异常增生的8个标本为上皮异常增生组;24周时处死另外20只,选取发生舌鳞癌的8个标本为鳞癌组。各组利用定量逆转录聚合酶链反应检测MMP-13在不同病变时期舌组织中转录水平的表达变化。结果大鼠诱癌16周时重度上皮异常增生发生率为75.0%(15/20),大鼠诱癌24周时舌鳞癌发生率为94.8%(18/19)。转录水平的检测显示MMP-13上皮异常增生组的表达较正常组上调,但差异无统计学意义(t=-1.759,P=0.129);舌鳞癌组与正常组(t=-6.579,P=0.001)和上皮异常增生组(t=-6.376,P=0.001)比较MMP-13的表达均有统计学意义的上调。结论 MMP-13随癌变的发生表达显著,可能在舌鳞癌的发生过程中起着重要作用。Objective To study the expression of transcription level of matrix metalloproteinases 13（MMP-13） in a rat model of tongue squamous cell carcinoma.Methods Sprague-Dawley rats were randomly divided into three groups.The experimental groups（16 rats）were given 4-nitroquinoline-1-oxide（4NQO） every day and the control group（8 rats） was given normal saline.Tongue samples were harvested from experimental rats when they were in epithelial dysplasia and tongue squamous cell carcinoma.The kinetics of MMP-13 expression was assayed by quantity reverse transcriptase-poly-merase chain reaction（qRT-PCR）.The paraffin-embeded tissue sections were made to observe the tissue pathological change.Results In normal rat tongue,a faint band for MMP-13 was observed by qRT-PCR.The expression of MMP-13 was increased in the tongue of the rats treatead with 4NQO in epithelial dysplasia,but was not statistically significant（t=-1.759,P=0.129）.And the tongue in squamous cell carcinoma rats showed a marked expression,and was statistically significant compared with epithelial dysplasia（t=-6.376,P=0.001） and the control rats（normal）（t=-6.579,P=0.001）.Conclusion The expression of MMP-13 may play an important role in the occurrence of tongue squamous cell carcinoma,and is more remarkable with the cancerous deep expression.

关 键 词：基质金属蛋白酶13 大鼠 舌鳞癌 

分 类 号：R739.86[医药卫生—肿瘤]