反义p53寡核苷酸对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响  

Effect of Antisense p53 Oligodeoxynucleotides on Adriamycin Inducing Apoptosis of Cultured Human Skin Keratinocytes in Vitro

作　　者：张兴洪[1] 刘彦群[1] 田美华[1] 

机构地区：[1]徐州医学院附属医院,徐州221002

出　　处：《中国中西医结合皮肤性病学杂志》2011年第3期141-144,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：江苏省中医药局中医药科研项目(H05103)

摘　　要：目的探讨反义p53寡核苷酸(ODN)对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响。方法用MTT法检测不同浓度脂质体和0.5mg/L反义p53 ODN转染角质形成细胞后对2mg/L阿霉素抑制细胞增殖的影响;RT-PCR方法测定p53 mRNA水平的变化;用流式细胞仪检测细胞凋亡。结果阿霉素抑制体外培养的人皮肤角质形成细胞增殖,5mg/L、10mg/L的脂质体和0.5mg/L反义p53 ODN转染细胞后,p53 mRNA水平下降(P<0.05),细胞增殖能力增强(P<0.05),凋亡率降低(P<0.05)。结论给予适当剂量反义p53 ODN转染人皮肤角质形成细胞,能一定程度减轻阿霉素对细胞的损伤,表现出对阿霉素致凋亡的保护作用。Objective To investigate the effect of antisense p53 oligodeoxynucleotides（ODN） on adriamycin inducing apoptosis of cultured human skin keratinocytes in vitro.Methods Human skin keratinocytes were cultured with 2mg/L adriamycin and transfected with 0.5mg/L antisense p53 ODN and different concentrations of liposome.Cell proliferation was detected by colorimetric assay of MTT.Then the optical density values were measured on a scanning multiwell spectrophotometer（ELISA reader）.The mRNA of p53 was also observed by RT-PCR after transfection with antisense p53 ODN.Apoptosis rate of human skin keratinocytes was detected by flow cytometry.Results We found that the proliferation of human skin keratinocytes was inhibited by adriamycin.If cells were incubated with 0.5mg/L antisense p53 ODN and 5mg/L or 10mg/L liposome,p53 mRNA decreased.A value was significantly higher and apoptosis induced by adriamycin was suppressed（P0.05）.Conclusion This study suggests that antisense p53 ODN could partially inhibit adriamycin inducing apoptosis of cultured human skin keratinocytes.

关 键 词：p53 反义寡核苷酸 阿霉素 角质形成细胞 细胞凋亡 

分 类 号：R96[医药卫生—药理学] R730.5[医药卫生—药学]