袖状胃切除术对高脂饮食诱导的肥胖大鼠主动脉内皮细胞LOX-1表达水平的影响  

Effect of Sleeve Gastrectomy on LOX-1 Expression of Aortic Epithelium in Rats with High-Fat-Diet-Induced Obestiy

作　　者：周勇[1] 王勇[1] 刘源[1] 耿东华[1] 赵文嫣[1] 刘金钢[1] 

机构地区：[1]中国医科大学附属盛京医院胆道疝减肥外科,辽宁沈阳110004

出　　处：《中国普外基础与临床杂志》2011年第6期615-619,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：辽宁省自然科学基金(项目编号:20102293)~~

摘　　要：目的探讨袖状胃切除术对于高脂饮食所诱导的营养性肥胖大鼠主动脉脂肪浸润及LOX-1表达的影响。方法 24只Wistar大鼠被随机分为正常喂养组(CO组)、高脂饮食组(HD组)及高脂饮食加袖状胃切除术组(SG组),其中术前CO组、HD及SG组均接受正常饮食。术后CO组接受正常饮食,HD组及SG组接受高脂饮食,分别于术后10、20及30 d检测大鼠体重变化。30 d时处死实验动物并应用ELISA法测定血浆高密度脂蛋白(HDL)和低密度脂蛋白(LDL)水平,Western blot法检查LOX-1水平,Real-time RT-PCR法检查LOX-1 mRNA表达,采用免疫组织化学方法经尼罗红染色检测主动脉内皮脂肪细胞浸润。结果 HD组大鼠术后体重明显高于另外两组(P<0.05)。CO组、HD组及SG组血浆HDL水平分别为(32.9±2.2)mg/dl(、43.4±1.4)mg/dl及(40.5±1.3)mg/dl,HD组明显高于CO组(P<0.05),HD与SG组差异无统计学意义。CO组、HD组及SG组血浆LDL水平分别为(31.8±1.6)mg/dl、(53.3±1.8)mg/dl及(37.5±1.5)mg/dl,HD组明显高于其他2组(P<0.01)。HD组的LOX-1蛋白和LOX-1 mRNA表达情况也明显高于另外两组(P<0.01)。HD组主动脉脂肪染色情况也提示HD组强于另外两组。结论高脂饮食可导致主动脉LOX-1蛋白和mRNA表达上升,袖状胃切除术后可使血浆LDL水平下降,从而使LOX-1蛋白和mRNA表达下调。Objective To investigate the effect of sleeve gastrectomy on preventing adipose infiltration and LOX-1 expression in aortic epithelium of high-fat-diet-induced obese rats.Methods Twenty-four Wistar rats were randomized into normal chow group（CO group）,high-fat diet group（HD group）,and high-fat diet combined with sleeve gastrectomy group（SG group）.Rats of three groups before operation and CO group after operation were fed with normal diet,rats of HD and SG group were fed with high-fat diet.Body weight of all the rats were examined on day 10,20,and 30 after operation,respectively.Animals were sacrificed on day 30 after operation and plasma HDL and LDL were detected by ELISA,LOX-1 and LOX-1 mRNA expression in aortic epithelium were measured by Western blot and real-time RT-PCR,respectively.Immunochemical histological Nile red stain was adopted in adipose infiltration examination of aorta.Results The body weights in HD group were much higher than those of other groups（P0.01）.The HDL in CO,HD,and SG groups was（32.9±6.2） mg/dl,（43.4± 4.0） mg/dl,and（37.5± 4.3） mg/dl,respectively.The LDL in CO,HD,and SG groups was（31.8±4.5） mg/dl,（53.3±5.1） mg/dl,and（40.5±3.7） mg/dl,respectively.The HDL and LDL values in HD group were higher than those of other two groups（P0.05,P0.01）.The LOX-1 protein and mRNA expressions in HD group were much higher than those of other groups（P0.01）.Adipose staining in HD group was also stronger than that of other groups.Conclusions High-fat diet can induce elevation of LOX-1 protein and mRNA expression in aorta.Sleeve gastrectomy can relieve plasma LDL level,as a result,LOX-1 protein and mRNA expression should be down-regulated.

关 键 词：袖状胃切除术 病态性肥胖 高脂饮食 主动脉 

分 类 号：R656.61[医药卫生—外科学] R622.9[医药卫生—临床医学]