镰刀菌发酵液对大豆胞囊线虫的抑制作用  

Inhibition of Fusarium.spp Fermented Filtrates on Soybean Cyst Nematode

作　　者：赵晓晖[1,2] 许艳丽[1] 

机构地区：[1]中国科学院东北地理与农业生态研究所黑土区农业生态院重点实验室,海伦农田生态系统国家野外观测研究站,黑龙江哈尔滨150081 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《大豆科学》2011年第3期468-470,474,共4页Soybean Science

基　　金：国家自然科学基金资助项目(30971900);黑龙江省科技攻关资助项目(GA09B103-10)

摘　　要：在大豆胞囊线虫(Heterodera glycines)自然衰退土壤中分离得到3株镰刀菌,F-9—禾谷镰孢菌(F.gra-minearum Schwabel),F-2、F-15—尖孢镰孢芬芳变种(F.oxysporum var.redolens(Wollenw)Gordon)。实验室条件下,研究了这3株镰刀菌发酵液原液对大豆胞囊线虫卵孵化及对二龄幼虫(J2)活性的影响。结果表明:F-2、F-9发酵液原液分别抑制了95.7%和95.2%的大豆胞囊线虫卵孵化,与对照组无菌水差异极显著;F-9发酵液原液在1 h时抑制J2活性高达90%,F-2发酵液原液在72 h时使全部的J2致死;F-15对J2不具有抑制性。F-2、F-15为同种的不同菌株,但是作用效果存在差异。In soybean cyst nematode suppressive soil,we isolated one F.graminearum（F-9） and two Fusarium oxysporumvar.redolens（F-2,F-15）.The objective was to determine the effects of Fusarium.spp fermented filtrates on second-stage juveniles（J2） and eggs of Heterodera glycines.The results showed that F-2、F-9 filtrates inhibited the nematode egg hatching by 95.67% and 95.17% respectively,it was extremely significant difference compared with water.F-9 filtrates were toxic to J2 up to 90% after 1 h.F-2 filtrates were toxic to 100% of J2.F-15 filtrates had no effect on J2.F-2 and F-15 were the same species,but had different effects.

关 键 词：禾谷镰孢菌 尖孢镰孢芬芳变种 发酵液 大豆胞囊线虫 生物防治 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]