培养基组成对里氏木霉合成纤维素酶的影响  

Effect of Medium Components on Cellulase Production by Trichoderma reesei

作　　者：李志和[1] 张丽[1] 李鑫[1] 朱均均[1] 余世袁[1] 勇强[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《林产化学与工业》2011年第3期55-59,共5页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(30871992;31070523);江苏省科技支撑计划项目(BE2010732);林业公益性行业科研专项项目(201004001);江苏高校科技创新团队项目资助(无编号)

摘　　要：研究了碳源种类及比例、氮源种类及比例、碳氮比和添加物等对里氏木霉合成纤维素酶的影响。里氏木霉以含纤维素质量比为4∶6的蒸汽爆破玉米秸秆和微晶纤维素为碳源,含氮素质量比为5∶2的硫酸铵-尿素为氮源,C/N为6,添加4 g/L黄豆粉和10 g/L麸皮为主要成分的培养基合成纤维素酶,纤维素酶活力在培养132 h达到最大,滤纸酶活力和β-葡萄糖苷酶活力分别为8.37 FPIU/mL和1.61 IU/mL。Effects of different kinds and ratio of carbon sources,nitrogen sources and C/N ratio on biosynthesis of cellulase by Trichoderma reesei Rut C-30 were investigated.Production of cellulase was carried out using steam-exploded corn stover and Avicel as carbon sources（m（corn stover）∶m（Avice）4∶6）,ammonium sulfate and urea as nitrogen sources（m（N of ammonium sulfate）∶m（N of urea）5∶2）,soybean meal 4g/L,wheat bran 10g/L and the C/N 6.The results showed that the maximum filter paper activity and β-glucosidase activity were 8.37FPIU/mL and 1.61IU/mL,after cultivation for 132 h respectively.

关 键 词：里氏木霉 纤维素酶 培养基 

分 类 号：TQ351[化学工程] Q814[生物学—生物工程]