X射线对人肺癌A549细胞CCR7表达影响的研究  

Effects of X-rays on CC-chemokine receptor 7 expression in human lung cancer A549 cells

作　　者：王翠兰[1] 江其生[2] 邹跃[2] 李峰生[2] 李伟[2] 宋秀军[2] 何蕊[2] 王璐[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院,215123 [2]中国人民解放军第二炮兵总医院

出　　处：《中华放射医学与防护杂志》2011年第3期299-302,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究不同剂量X射线照射及照射后不同时间点对人肺癌A549细胞CC-趋化因子受体7（CCR7）表达的影响。方法体外培养A549细胞，实验组采用直线加速器X射线一次性照射，细胞吸收剂量分别为2、4、6和8Gy（源皮距100em；剂量率442．89cGy／min），照射后4、12、24、48和72h分别采用实时荧光定量PCR技术及Westernblot方法分别进行CCR7mRNA及蛋白质表达水平检测；对照组A549细胞除不接受X射线照射外，余处理同实验组。结果A549细胞经2、4、6和8Gy的X射线照射后，CCR7mRNA及蛋白质在照射4h后开始表达升高，达到高峰后相继出现下降；72h后6和8Gy组mRNA表达量仍高于对照组水平（t=6．75—7．26，P〈0．01），2和6Gy组蛋白质表达量高于对照组（t=11．13—14．17，P〈0．01），而4和8Gy组蛋白质表达量在48和72h已降至对照组水平。结论2、4、6和8Gy的X射线照射A549细胞后，A549细胞CCR7mRNA及蛋白质的表达量明显增加，可能与一定剂量X射线辐射促进A549细胞增殖和转移有关。Objective To study the effects of X-ray radiation on CC-chemokine receptor 7 （CCR7） expression in human non-small cell lung cancer （NSCLC） cells. Methods Human adenocareinoma cells of the line A549 were cultured and irradiated by X-ray at the absorbed doses of 2, 4, 6, and 8 Gy respectively by linear accelerator （with the source skin distance of 100 cm and close rate of 442.89 eGy/min）. The relative levels of CCR7 mRNA and protein expression in the A549 cells were respectively detected by real time-PCR and Western blotting 4, 12, 24, 48, and 72 h after radiation, Untreated A549 cells were used as control group. Results The expression levels of CCR7 mRNA and protein in the A549 cells began to increase since 4 h after radiation and then decreased gradually after they reached the peak. The CCR7 mRNA expression levels 72 h after radiation of the 6 and 8 Gy groups were still significantly higher than those of the control group （t =6.75 -7.26,both P 〈0. 01 ） , and the CCR7 protein expression levels of the 2 and 6 Gy group were still significantly higher than those of the control group（t=11.13- 14.17, both P 〈0.01）. Then the CCR7 protein expression levels of the 4 and 8 Gy groups decreased to the control group level 48 ancl 72 h after radiation respectively. Conclusions The CCR7 mRNA and protein expression levels in the NSCLC cells increase after X-ray irradiation, which may be correlated with the promotion of proliferation and metastasis of NSCLC cells by X-ray irradiation at a certain dose.

关 键 词：肺癌 CC-趋化因子受体7 X射线 

分 类 号：R734.2[医药卫生—肿瘤]