非小细胞肺癌组织中TUBB3基因的表达及临床意义  

TUBB3 GENE EXPRESSION AND CLINICAL SIGNIFICANCE IN NON-SMALL CELL LUNG CANCER

作　　者：冷雪峰[1] 冼磊[1] 陈铭伍[1] 李满红[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁530021

出　　处：《广西医科大学学报》2011年第3期378-381,共4页Journal of Guangxi Medical University

基　　金：广西科学研究与技术开发计划项目(No.桂科攻10124001A-44);广西研究生创新教育项目(No.2010105981002M181);广西壮族自治区大型仪器网测试资助项目

摘　　要：目的:探讨TUBB3基因在非小细胞肺癌(NSCLC)组织中的表达及临床意义。方法:采用逆转录—聚合酶链反应(RT-PCR)方法检测82例肺癌组织、36例癌旁组织及15例良性组织中TUBB3基因mRNA的表达。结果:TUBB3基因在NSCLC癌、癌旁及良性组织中表达分别为18.3%(15/82)、2.8%(1/36)及93.3%(14/15)。3者比较后总体差异有统计学意义(P<0.001)。癌组织TUBB3表达18.3%高于癌旁组织2.8%(P=0.048);腺癌TUBB3表达25.9%(14/54)高于鳞癌3.6%(1/28)(P=0.013)。结论:TUBB3基因mRNA癌组织中表达高于癌旁组织,尤其是腺癌表达高于鳞癌可指导广西人群患者术后紫杉醇类药物的个体化治疗。Objective：To investigate the expression and clinical significance of TUBB3 gene mRNA in nonsmall cell lung cancer（NSCLC）. Methods：Using reverse transcription- polymerase chain reaction （RT-PCR） detected 82 cases of lung cancer, 36 cases of adjacent tissues and 15 cases of benign tissue mRNA expression of TUBB3 gene. Results：The expression of TUBB3 gene in NSCLC patients, adjacent and benign tissues was 18.3%（15/82）, 2.8%（1/36） and 93.3%（14/15, P〈0.001）; respectively. The expression of TUBB3 in tumor was than higher that in adjacent tissues（ P =0. 048）. The expression of TUBB3 in adenocarcinoma were also higher than that in squamous cell carcinoma （25.9% vs 3.6%, P =0. 013）. Conclusion. TUBB3 gene mRNA expression in tumor is higher than in adjacent tissues, especially in adenocarcinoma than in squamous cell carcinoma. It can guide the Guangxi population individual treatment of paclitaxel.

关 键 词：非小细胞肺癌 TUBB3基因 逆转录-聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]