济南汉族群体5个Y-SNP位点的多态性分析  

Polymorphic Analysis of 5 Y-SNP Loci in Han Population of Jinan

作　　者：韩淑毅[1] 高洪梅[1,2] 张茂修[1,2] 郑燕[1] 郏雁飞[1] 丁卜同[1] 汪运山[1,2] 

机构地区：[1]山东大学附属济南市中心医院,山东济南250013 [2]济南迪恩法医司法鉴定所,山东济南250013

出　　处：《法医学杂志》2011年第3期205-207,210,共4页Journal of Forensic Medicine

基　　金：济南市科技局项目(066007)

摘　　要：目的探讨济南汉族群体5个Y-SNP位点的多态性并评价其在法医学中的应用。方法采用片段长度差异等位基因特异性PCR（fragment length difference allele specific PCR,FLDAS-PCR）对济南汉族群体共103名男性无关个体5个Y-SNP标记（M89、M9、M122、M134、M95）进行分型研究。结果 5个Y-SNP位点在济南汉族群体中均呈多态性分布,基因多样性（GD）为0.093 3~0.491 2,共检测到20种单倍型（HD为0.8679）,根据国际Y染色体协会命名原则,共有6种单倍群。结论 5个Y-SNP位点及其单倍型在济南汉族具有较好的多态性分布,为该地区人群遗传结构的分析及法医遗传学研究提供了较多的信息量。Objective To investigate polymorphism distribution of the 5 Y-SNP loci in Jinan Han population,and evaluate their potential in forensic application.Methods Genotyping of 5 Y-SNP loci（M89,M9,M122,M134,M95） were executed in the sample of 103 unrelated Chinese male individuals in Jinan Han population by using fragment length discrepant allele specific PCR（FLDAS-PCR）.Results In 5 Y-SNP loci,genetic polymorphism were identified in Jinan Han population,and the ranges of gene diversity（GD） were 0.093 3-0.491 2.Twenty different haplotypes were observed and the haplotypes diversity（HD） was 0.867 9.Six different haplogroups were detected according to international association of Y chromosome nomenclature.Conclusion Five Y-SNP loci and their haplogroups in Jinan Han population are highly polymorphic,which can provide more information for the genetic structure analysis and forensic genetics research in the region.

关 键 词：法医遗传学 多态性 单核苷酸 Y染色体 汉族 

分 类 号：R394[医药卫生—医学遗传学]