去整合素kistrin干预兔后发性白内障形成中晶状体上皮细胞FAK及ERK基因表达的研究  

Gene expressions of FAK and ERK in lens epithelial cells on rabbit posterior capsular opacification models with kistrin interference

作　　者：于美沁[1] 谭少健[1] 陈琼[1] 梁皓[1] 何剑峰[1] 李霞[1] 黄敏丽[1] 陈金卯[1] 陈迎迎[1] 

机构地区：[1]广西医科大学一附院眼科中心,广西壮族自治区南宁市530021

出　　处：《眼科新进展》2011年第6期504-507,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30760268);广西自然科学基金资助(桂科自:0833141)~~

摘　　要：目的研究去整合素kistrin对兔后发性白内障模型晶状体上皮细胞中黏着斑激酶(focal adhesion kinases,FAK)mRNA和其下游因子细胞外信号调节激酶(extracellular signal regulated kinase,ERK)mRNA表达的影响,以探讨kistrin降低兔后发性白内障形成的可能分子机制。方法 8只新西兰大白兔均行右眼透明晶状体囊外摘出术(extracapsular lens extraction,ECLE)建立后发性白内障模型。随机将8只术眼分为实验组和对照组(每组4眼),术毕实验组囊袋内注入80μg.L-1kistrin0.2mL,对照组注入等量林格氏液。随机选4眼左眼为空白组。术后14d取残留的晶状体囊袋行RT-PCR检测FAK和ERK mRNA的表达。结果正常兔晶状体上皮细胞中(空白组)FAK和ERKmRNA表达量少,分别为1.476±0.802、0.576±0.035。ECLE术后14d对照组FAK和ERK mRNA表达量分别为6.496±1.501、1.158±0.573,实验组分别为6.060±1.512、1.262±0.329,2组表达量均较空白组显著性升高,差异均有统计学意义(均为P<0.05),实验组与对照组比较差异均无统计学意义(均为P>0.05)。结论兔后发性白内障形成的早期,FAK及ERK基因表达上调。应用kistrin未改变此两基因在转录水平上的表达。Objective To evaluate the gene expressions of focal adhesion kinases（FAK）mRNA and extracellular signal regulated kinase（ERK）mRNA in the lens epithelial cells on the rabbit posterior capsular opacification（PCO）models with kistrin interference and investigate the possible mechanisms of this procedure.MethodsExtracapsular lens extraction was performed on right eyes of 8 New Zealand white rabbits to establish the PCO model.Eight right eyes were randomly divided into control group and experimental group,4 eyes in each group.At the end of the operation,0.2 mL kistrin（80 μg·L-1）were injected into the capsular bags of the experimental group,while 0.2 mL Ringer＇s irrigation were injected into the control group.Four left eyes were chosen randomly and worked as the blank group.The residual lens capsules were taken out at the postoperative 14th day.The mRNA of posterior capsules of the cells from 3 groups were extracted and the mRNA expressions of FAK and ERK were evaluated by real-time PCR.ResultsThe normal rabbit residual lens capsules expressed few FAK mRNA and ERK mRNA,which were 1.476±0.802 and 0.576±0.035.At the 14th day after surgery,the expressions of FAK mRNA and ERK mRNA were 6.496±1.501 and 1.158±0.573 in control group,6.060±1.512 and 1.262±0.329 in experimental group.The expressions of FAK mRNA and ERK mRNA increased significantly in the control group and the experimental group compared with the blank group（all P0.05）.There was no difference between the experimental group and the control group（all P0.05）.ConclusionsThe lens epithelial cells express more mRNA of FAK and ERK in the early stage of PCO.Administration of kistrin can not change gene expression of FAK and ERK.

关 键 词：去整合素 黏着斑激酶 细胞外信号调节激酶 基因表达 信号转导通路 

分 类 号：R776.1[医药卫生—眼科]