青光眼模型家兔血浆β-内啡肽浓度与眼压关系的研究  

Research on relationship between concentration of β-endorphin in plasma and IOP in rabbit with glaucoma

作　　者：孙红[1,2] 袁志兰[2] 王育良[1] 范莲[2] 罗莎莎[2] 

机构地区：[1]南京中医药大学眼科,江苏省南京市210029 [2]南京医科大学第一附属医院眼科,江苏省南京市210029

出　　处：《眼科新进展》2011年第7期615-617,共3页Recent Advances in Ophthalmology

基　　金：江苏省教育厅科学基础研究项目(编号:07KJB310070)~~

摘　　要：目的探讨青光眼模型家兔血浆β-内啡肽浓度与眼压的关系。方法 采用前房内注射20 g.L-1甲基纤维素制作家兔青光眼模型,共6只。造模成功后4 h、8 h、24 h进行眼压测定,采用酶联免疫检测方法测定血浆β-内啡肽浓度。结果 6只家兔眼压值造模前,造模后4 h、8 h、24 h分别为(7.61±1.68)mmHg(1 kPa=7.5 mmHg)、(18.83±8.01)mmHg、(12.72±5.30)mmHg、(10.22±5.01)mmHg。血浆β-内啡肽浓度造模前为(22.97±7.68)μg.L-1,造模后4 h、8 h、24 h分别为(49.20±21.94)μg.L-1、(39.30±8.56)μg.L-1、(32.18±11.92)μg.L-1。造模后4 h、8 h眼压值与血浆β-内啡肽浓度与造模前比较,差异均有统计学意义(均为P<0.05),造模后24 h眼压值与血浆β-内啡肽浓度与造模前比较差异均无统计学意义(均为P>0.05)。血浆β-内啡肽浓度与眼压水平呈正相关(P<0.05)。结论 血浆中β-内啡肽浓度的异常变化可能是青光眼眼压调控机制中的一个重要环节。Objective To study the relationship between the concentration of β- endorphin in plasma and intraocular pressure （IOP） in rabbit with glaucoma. Methods Methyl cellulose was injected into the anterior chamber of rabbit eyes to induce glaucoma. Prior to injection and at 0 hour,4 hours,8 hours and 24 hours after injection,IOP was monitored and β-endorphin concentrations in plasma of rabbits were measured with enzyme linked immunosorbent assay. Results Prior to injection and at 4 hours,8 hours and 24 hours after injection, the IOP of rabbits were （ 7. 51 ± 1. 68 ） mmHg （1 kPa=7.5 mmHg） ,（18.83±8.01） mmHg,（12.72 ±5.30）mmHg and （10.22 ±5.01） mmHg,respectively. And β-endorphin concentrations in plasma were （22.97 ± 7.68 ） μg· L-1,（49.20±21.94）μg· L-1,（39.30±8.56）μg· L-1,（32.18-±ll.92）μg· L-1,respectively. Compared with that prior to injection, the IOP and β-endorphin concentrations in plasma was obviously increased at 4 hours and 8 hours after injection （ all P 〈 0. 05） ,while no difference was found at 24 hours after modeling. The β-endorphin concentrations in plasma was positively correlated with IOP. Conclusion Abnormal changes of β-endorphin concentration may play an important role in the control of IOP for glaucoma.

关 键 词：Β-内啡肽 青光眼模型 兔 

分 类 号：R775[医药卫生—眼科]