Q开关YAG激光及化学脱色法构建色素脱失动物模型的比较  

The Difference between the Two Methods of Animal Models for Depigmentation Separately Induced by Q-Switched YAG Laser and Chemical Decolorization

作　　者：张雪静[1] 刘莉萍[1] 马红[1] 许辉[1] 顾文涛[1] 李遇梅[1] 

机构地区：[1]江苏大学附属医院皮肤科,江苏镇江212001

出　　处：《中国皮肤性病学杂志》2011年第7期530-534,共5页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(30972663);中华医学会皮肤性病学分会-安斯泰来皮肤病学研究基金资助

摘　　要：目的探讨Q开关YAG激光及化学脱色法对豚鼠皮肤黑素细胞的影响,从而构建色素脱失动物模型。方法分别应用Q开关YAG激光照射及5%H2O2化学脱色处理豚鼠黑色皮肤,肉眼观察皮肤颜色变化,HE染色和HMB45免疫组化染色观察皮肤黑素细胞的变化。结果 Q开关YAG激光照射3次后,豚鼠黑色皮肤变为白色,同时长出白色毛发,HE及HMB45染色后基底层未见黑素细胞。2个月后,照射区域皮肤及毛发仍呈白色,镜下基底层亦未见黑素细胞。而化学脱色法可使皮肤略变白,毛发仍呈黑色,HE及HMB45染色镜下基底层仍可见大量黑素细胞。结论 Q开关YAG激光可以构建短期稳定色素脱失动物模型,为色素减少性皮肤病的研究提供了可依据的动物模型。Objective To explore the changes on melanocytes from guinea pig skin which was derived by Q-Switched YAG laser or chemical decolorization and to establish animal models for depigmentation. Methods Q- Switched YAG laser or 5% H202 chemical decolorization was separately applied to guinea pig＇s dark skin, and then the change of skin color was observed under macroscopy while melanocytes were detected after stained by HE and immunohistochemistry with HMB45. Results After radiated by Q-Switched YAG laser for three times, the dark skins became white and grew white hair, meanwhile melanocytes were not seen in the stratum basale through HE and HMB 45 stain. Two months later, the skins and hair still maintained white and melano- cytes couldn＇t be seen in the stratum basale. However, chemical decolorization could slightly make darken skins decolored while the hair still appeared black, and melanocytes could be seen obviously after stained with HE and HMB45. Conclusion Compared with chemical decolorization, Q-Switched YAG laser could establish stable animal models for depigmentation temporarily.

关 键 词：Q开关YAG激光 化学脱色法 黑素细胞 动物模型 

分 类 号：R364.23[医药卫生—病理学]