^(18)氟-氟代脱氧葡萄糖诱导食管癌Eca-109细胞凋亡的初步研究  

The apoptosis of esophageal Eca-109 cells induced by ^(18)F-2-deoxy-2-fluoro-D-glucose

作　　者：朱梅[1] 王明明[1] 邱俊[1] 吴翠萍[1] 谢强 谢吉魁 

机构地区：[1]安徽医科大学核医学教研室,合肥230032 [2]徽医科大学附属省立医院PET-CT中心,合肥230001

出　　处：《安徽医科大学学报》2011年第7期619-623,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金(编号:KJ2011Z162);中科院新型薄膜太阳能电池重点实验室开放基金(编号:2010007)

摘　　要：目的探讨正电子药物18氟-氟代脱氧葡萄糖(18F-FDG)诱导食管癌细胞Eca-109凋亡过程中的作用及其可能机制。方法用不同活度浓度的18 F-FDG处理Eca-109细胞,应用MTT法检测18F-FDG对Eca-109细胞增殖的影响;流式细胞仪检测细胞凋亡情况,DCFH-DA法检测细胞内活性氧(ROS)水平的变化,FCM检测细胞内线粒体膜电位(△Ψm)水平变化情况。结果 18 F-FDG可明显抑制Eca-109细胞增殖;0～74 MBq/ml的18F-FDG作用于Eca-109细胞6 h后,细胞内ROS水平分别为(13.13±2.90)%、(19.07±1.40)%、(23.93±3.18)%、(66.23±5.94)%、(78.13±1.96)%,24 h后Δψm水平分别为(98.03±1.68)%、(92.77±2.27)%、(77.80±2.62)%、(68.27±4.86)%、(34.83±2.86)%,48 h后可见细胞凋亡率分别为(0.97±0.28)%、(5.2±0.44)%、(10.5±1.08)%、(19.37±3.98)%、(39.87±3.51)%。结论正电子药物18F-FDG可通过产生β+粒子对Eca-109食管癌细胞进行内照射,产生ROS,引发线粒体膜电位变化来诱导细胞凋亡,且凋亡率随药物的活度浓度增大而增大。Objective To investigate the effects of 18F-FDG on apoptosis of Eca-109 cells and its possible mechanisms.Methods Eca-109 cells were conventionally cultered and treated with 0~ 74MBq/ml of 18F-FDG at 24 h after culturing.MTT assay were used to analyse the proliferation of Eca-109 cells.The apoptosis,levels of reactive oxygen species（ROS） in cells and the mitochondrial membrane potential（△Ψm） were detected by flow cytometry.Results 18F-FDG could significantly inhibit the proliferation of Eca-109 cells.At 6 h after treated with 0~74MBq/ml of 18F-FDG,ROS levels were（13.13±2.90）%,（19.07±1.40）%,（23.93±3.18）%,（66.23±5.94）%,（78.13±196）%,respectively;at 24 h,Δ ψ m were（98.03±1.68）%,（92.77±2.27）%,（77.80±2.62）%,（68.27±4.86）%,（34.83±2.86）%,respectively;at 48h,apoptotic rates of cell were（0.92±0.28）%,（5.2±0.44）%,（10.5±1.08）%,（19.37±3.98）%,（39.8±3.51）%,respectively.Conclusion 18F-FDG generates ROS in Eca-109 cells,which can cause mitochondrial membrane permeated and Δψm decreased,therefore,induces to apoptosis of Eca-109 cells,and apoptosis rate increases with the concentration of 18F-FDG.

关 键 词：氟脱氧葡萄糖F18 食管肿瘤 活性氧 膜电位 线粒体 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤] R329.25[医药卫生—临床医学]