1,25二羟维生素D3和塞莱昔布增强阿霉素对乳腺癌细胞株MCF/ADR的作用与NF-κB的关系  

The effect of 1α,25-dihydroxyvitamin D3 associated with celecoxib enhanced the role of doxorubicin on breast cancer cell line MCF/ADR via NF-κB

作　　者：曹永一 鲍扬漪 

机构地区：[1]安徽医科大学第三附属医院血液肿瘤科,合肥230031

出　　处：《安徽医科大学学报》2011年第7期631-635,共5页Acta Universitatis Medicinalis Anhui

摘　　要：目的研究1,25二羟维生素D3(1,25-D)和塞莱昔布(CELE)影响阿霉素(ADM)对MCF/ADR细胞的作用与NF-κB的关系。方法采用四唑氮蓝比色(MTT)法检测ADM的半数药物抑制浓度(IC50);采用流式细胞技术分析1,25-D和CELE对MCF/ADR细胞凋亡、细胞内ADM累积、P170表达的影响;免疫组化SP法检测1,25-D和CELE作用于MCF/ADR细胞后P65、IκBα表达水平。结果 1,25-D(50×10-9 mol/L)联合CELE(25×10-6 mol/L)和ADM作用后,MCF/ADR细胞凋亡率升高(P<0.01),ADM的IC50显著降低(P<0.01),P65和P170蛋白表达水平降低,IκBα表达增高(P<0.01)。结论 1,25-D联合CELE通过抑制NF-κB的活化、促进细胞凋亡从而增强ADM对耐药细胞的作用。Objective To study the effect of 1α,25-dihydroxyvitamin D3 associated with celecoxib affected doxorubicin on breast cancer cell line MCF/ADR via NF-κB.Methods MTT assay was used to detect MCF/ADR cells proliferation and half inhibitory concentration（IC50） of doxorubicin.MCF/ADR cell apoptosis,intracel-lular concentration of doxorubicin and cell surface P170 expression were determined by flow cytometry.The expression of P65 and IκBα was examined by immunohistochemistry.Results Administration of 1α,25-dihydroxyvitamin D3 50×10-9 mol/L and celecoxib 25×10-6 mol/L together inhibited MCF/ADR cells proliferation,induced cell apoptosis and increased cell sensitivity to doxorubicin.In addition,combination of these two drugs decreased P65 and P170 expression but increased IκBα expression in MCF/A-DR cells.Conclusion 1α,25-dihydroxyvitamin D3 associated with celecoxib enhanced doxorubicin sensitivity of MCF/ADR cells which probably through inhibiting NF-κB and promoting cell apoptosis.

关 键 词：乳腺癌 NF-ΚB 1 25二羟维生素D3 塞莱昔布 

分 类 号：R737.9[医药卫生—肿瘤] R977.24[医药卫生—临床医学]