脂肪酸合成在利福平引起小鼠肝脏脂肪变性中的作用  

The role of synthesis of fatty acids on rifampicin arosing the steatosis in mice liver

作　　者：张莹[1] 段自皞[1] 陈熙[1] 张程[1] 徐德祥[1] 

机构地区：[1]安徽医科大学卫生毒理学系,合肥230032

出　　处：《安徽医科大学学报》2011年第7期648-651,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30572223;81001480);安徽省自然科学基金(编号:090413142)

摘　　要：目的研究利福平(RIF)引起小鼠肝脏发生脂肪变性的作用机制。方法将雌性ICR成年健康小鼠随机分为对照组和RIF组,分别灌胃给予等量的生理盐水或RIF(200mg/kg),连续处理1周及单次处理。分析血清和肝脏中甘油三酯(TG)水平,油红O染色观察肝脏脂肪聚集情况,RT-PCR法检测肝脏脂肪酸合成酶(Fasn)、乙酰辅酶A羧化酶(Acc)、硬脂酰辅酶A去饱和酶1(Scd-1)和清道夫受体(CD36)水平。结果油红O染色病理观察发现RIF 1周引起肝脏脂肪变性,RIF单次引起轻微脂质聚集。RIF 1周和单次处理小鼠血清TG水平与对照组相比无明显变化,肝脏组织中TG水平显著升高。1周和单次RIF处理小鼠肝脏组织Fasn、Acc和CD36 mRNA水平均明显上调,Scd-1 mRNA水平无明显变化。结论 RIF影响小鼠肝脏脂质代谢,脂肪酸合成和转运的关键酶在RIF干扰脂质代谢中起重要作用。Objective To investigate the mechanism of rifampicin（RIF） arose steatosis in mouse liver.Methods ICR female mice were divided into the control group and the RIF group.Who were respectively administered with saline or RIF（200 mg/kg）daily by gavage for one week or a single day.Assays for triglyceride（TG）was performed to assess liver steatosis.The lipid accumulation of liver was observed by oil red O staining.The expressions of fatty acid synthetase（Fasn）,acetyl-CoA catboxylase（Acc）,stearoyl-CoA desaturase 1（Scd-1）and free fatty acid transporter（CD36）in liver were determined by RT-PCR.Results Steatosis was observed in mice liver after one week of RIF.Slight lipid accumulation was observed in mice liver after a single day of RIF.Compared with the control group,the RIF group one week or a single dose showed a remarkable enhancement in TG in mice liver.RIF significantly up-regulated the expression of Fasn,Acc and CD36 in mice liver,and there is no significant difference about Scd-1.Conclusion RIF may arose steatosis in mice liver via up-regulating the expression of key enzyme in fat synthesis and transport.

关 键 词：利福平 肝 脂肪酸类 

分 类 号：R364.24[医药卫生—病理学] R344.7[医药卫生—基础医学]