GW3965在Raji细胞中对BLyS表达的影响  

Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells

作　　者：黄艳[1] 黄刚[2] 胡长江[2] 曾益军[2] 许志臻[2] 陈岺曦[2] 何凤田[2] 宋方洲[1] 

机构地区：[1]重庆医科大学分子医学与肿瘤研究中心,重庆400016 [2]第三军医大学基础医学部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2011年第13期1342-1345,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(81001302)~~

摘　　要：目的探讨肝X受体(liver X receptors,LXRs)的特异性激动剂GW3965在Raji细胞中对B淋巴细胞刺激因子(B lymphocyte stimulator,BLyS)表达的影响。方法 MTT法检测不同浓度的GW3965(浓度分别为0.5、5、10μmol/L)对Raji细胞活性的影响,观察时间为加入GW3965共孵育后24 h。用LXR的特异性激动剂GW3965刺激人B细胞淋巴瘤株Raji细胞,经RT-PCR检测LXR特异性靶基因三磷酸腺苷结合盒A1(ATP-binding cassette,ABCA1)mRNA的表达;经RT-PCR和Western blot检测BLyS的表达。结果对照组1‰DMSO D(492)为(0.632±0.055),0.5、5μmol/L和10μmol/L的GW3965 D(492)为(0.609±0.073)、(0.612±0.052)和(0.628±0.055)。LXR的特异性配体GW3965对细胞活性无影响(P>0.05)。GW3965作用于Raji细胞后,ABCA1 mRNA表达剂量依赖性上调,LXR活化后可在转录和翻译水平剂量依赖性下调抑制BLyS的表达。结论 LXR在Raji细胞中可抑制BLyS的表达。Objective To determine the effect of liver X receptor（LXR） specific agonist,GW3965 on the expression of B lymphocyte stimulator（BLyS） in human B-cell lymphoma Raji cells.Methods Cell viability of Raji cells exposure to GW3965 at concentrations of 0.5,5 and 10 μmol/L for 24 h was detected by MTT assay.Then the expression of ATP-binding cassette（ABCA1,the specific target gene of LXR） mRNA and LXR mRNA was detected by RT-PCR and the expression of BLyS at mRNA and protein levels were measured by RT-PCR and Western blotting.Results The level of 1‰ DMSO OD（492 nm） was（0.632±0.055）,and 0.5,5 and 10 μmol/L GW3965 resulted in the levels of OD（492nm） were（0.609±0.073）,（0.612±0.052） and（0.628±0.055） respectively,indicating that GW3965 had no effect on the viability of Raji cells（P〉0.05）.Exposure to 0.5 and 5 μmol/L GW3965 results in an increase in the level of ABCA1 mRNA in a dose-dependent manner,suggesting LXR was functional in Raji cells.The expression of BLyS were down-regulated in both mRNA and protein levels after LXR activation.Conclusion LXR decreases the expression of BLyS in Raji cells.

关 键 词：GW3965 B淋巴细胞刺激因子 RAJI细胞 

分 类 号：R394[医药卫生—医学遗传学] R73-362[医药卫生—基础医学]