桩蛋白表达下调对结直肠癌细胞SW 480侵袭力的影响  

shRNA-mediated down-regulation of paxillin reduces cell invasion in human colon adenocarcinoma cell line SW480

作　　者：张全文[1] 汪欣[1] 万远廉[1] 刘玉村[1] 朱静[2] 

机构地区：[1]北京大学第一医院普外科,北京市100034 [2]北京大学第一医院外科实验室,北京市100034

出　　处：《世界华人消化杂志》2011年第16期1693-1697,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨下调桩蛋白(paxillin)的表达对结直肠癌细胞SW480体外侵袭的影响.方法:筛选常见结直肠癌细胞株中paxillin的表达,发现SW480中表达最多.构建干扰paxillin表达的特异性短发夹RNA(shRNA),转染结直肠癌细胞SW480.将SW480细胞分为3组:正常SW480组、阴性对照组及paxillin shRNA组,通过侵袭小室实验观察各组细胞侵袭力的变化.结果:转染paxillin shRNA后,结直肠癌细胞SW480中paxillin的表达明显降低.正常SW480组、阴性对照组及paxillin shRNA组穿透细胞数分别为62.00±6.26,55.00±13.04和23.33±6.12,三组差异具有显著性(F=30.976,P<0.05).结论:下调结直肠癌细胞SW480中paxillin蛋白表达可使细胞体外侵袭能力降低.AIM： To investigate the effect of short hairpin RNA （shRNA）-mediated down-regulation of paxillin expression on cell invasion in human colorec- tal adenocarcinoma cell line SW480 in vitro. METHODS： shRNA targeting the paxillin gene was constructed and transfected into SW480 cells. SW480 cells were divided into three groups： untransfected cells, cells transfected with a control shRNA, and those transfected with a paxillin-specific shRNA. After transfection, the invasion of cells was analyzed by Tran- swell migration assay. RESULTS： The expression of paxillin was inhibited in SW480 cells after the transfection ofpaxillin-specific shRNA. The numbers of cells passing the Transwell membrane were significantly lower in cells transfected with the paxillin-specific shRNA than in untransfected cells and those transfected with control shRNA （23.33 ± 6.12 vs 62.00 ± 6.26, 55.00 ± 13.04, F = 30.976, P 0.05）. CONCLUSION： Down-regulation of paxillin gene expression reduces cell invasion in human colon adenocarcinoma cell line SW480 in vitro.

关 键 词：桩蛋白 短发夹RNA SW480细胞株 细胞侵袭能力 

分 类 号：R735.3[医药卫生—肿瘤]