灵芝孢子粉对癫痫大鼠脑组织细胞色素C、线粒体钙、HSP70和BDNF的影响  

Effect of Ganoderma lucidum spores on cytochrome C,mitochondrial Ca^(2+),HSP70 and BDNF in brain of epilepsy rats

作　　者：王淑秋[1,2] 李晓捷[3,2] 姜志梅[3,2] 秦晓玉[4] 韩玉泽[1] 刘蕾[1] 刘君星[1] 王芳芳[1] 梁衍峰[1] 王淑湘[1] 

机构地区：[1]佳木斯大学基础医学院病理生理学教研究室,黑龙江佳木斯154007 [2]佳木斯大学儿童神经康复重点实验室,黑龙江佳木斯154007 [3]佳木斯大学康复医学院,黑龙江佳木斯154007 [4]佳木斯大学附属第一医院,黑龙江佳木斯154007

出　　处：《中国病理生理杂志》2011年第6期1053-1058,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81041116);黑龙江省自然科学基金资助项目(No.200803)

摘　　要：目的:研究灵芝孢子粉对癫痫大鼠脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)、细胞色素C、热休克蛋白70(HSP70)、线粒体Ca2+和脑源性神经营养因子(BDNF)的影响,探讨灵芝孢子粉对癫痫大鼠脑细胞的保护作用机制。方法:用亚惊厥剂量的戊四氮(PTZ)复制癫痫慢性模型,采用火焰原子吸收法测定脑组织线粒体Ca2+的含量,分光光度计比色法测定SOD的活性、MDA和细胞色素C含量,免疫组织化学方法检测HSP70和BDNF的表达情况。结果:与癫痫模型组相比,灵芝孢子粉组线粒体内细胞色素C、线粒体Ca2+的含量和HSP70的表达显著增高,而胞浆内细胞色素C含量则明显降低;脑组织SOD和T-AOC的活性明显增加,而MDA含量明显降低。大脑皮质和海马区BDNF阳性细胞数癫痫模型组明显高于正常对照组(P<0.05);灵芝孢子粉组皮质和海马区BDNF阳性神经元数目进一步增多,与癫痫模型组相比,差异显著(P<0.05)。结论:灵芝孢子粉能明显降低癫痫发作对脑细胞线粒体造成的损伤,其作用机制可能是通过清除自由基、增强脑组织的抗氧化能力,从而减轻自由基对线粒体膜的损伤作用,恢复线粒体的能量代谢,减轻脑细胞的损伤与凋亡。AIM：To investigate the effects of Ganoderma lucidum spores on superoxide dismutase（SOD）,malondialdehyde（MDA）,total antioxidative capacity（T-AOC）,cytochrome C,heat-shock protein 70（HSP70）,mitochondrial Ca2＋ and brain-derived neurotrophic factor（BDNF） in the brain tissues of epilepsy rats.METHODS： The rat chronic epilepsy model was by intraperitoneal injection of pentetrazole（PTZ） at a subconvulsant dose（32 mg/kg）.Flame atomic absorption method was used to detect the content of mitochondrial Ca2＋,and spectrophotometer colorimetry was used to measure SOD activity,MDA content,T-AOC and cytochrome C levels in rat brain tissues. HSP70 and BDNF were determined by immunohistochemical method.RESULTS： The contents of mitochondrial Ca2＋ and cytochrome C were higher,and the content of intracytoplasmic cytochrome C in the rat brain tissues was obviously lower in Ganoderma lucidum spores group than that in epileptic model group.Compared to epileptic model group,the activity of SOD and T-AOC in cytoplasm of the rat brain tissues decreased while MDA increased,and the numbers of BDNF-positive cells in cerebral cortex and hippocampus were significantly increased in Ganoderma lucidum spores group.The positive neuron population of HSP70 in hippocampus,basal nucleus and cortex was significantly higher in Ganoderma lucidum spores group than that in epilepsy model group.CONCLUSION： Ganoderma lucidum spores attenuate the impairment of neuronal mitochondria induced by seizure,and accelerate the expression of BDNF,resulting in restoring the energy metabolism in mitochondrion,thus alleviating the impairment and apoptosis of the brain tissues.

关 键 词：癫痫 灵芝孢子粉 线粒体钙 热休克蛋白70 脑源性神经营养因子 

分 类 号：Q95-3[生物学—动物学] R742.1[医药卫生—神经病学与精神病学]