姜黄素衍生物B50对不同辐射抗拒的同源鼻咽癌细胞增殖及凋亡的作用  

Effects of curcumin analogue B50 on proliferation and apoptosis of homologous nasopharyngeal carcinoma cells with different radioresistance

作　　者：刘嘉伟[1] 张剑威[1] 杨广鑫[1] 陈淑英[1] 方乐堃[1] 梁广[2] 肖健[2] 杨惠玲[1] 

机构地区：[1]中山大学中山医学院,广东广州510080 [2]温州医学院药学院,浙江温州325000

出　　处：《中国病理生理杂志》2011年第6期1077-1083,共7页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670627);广东省科技计划资助项目(No.2008A030201009);广州市科技计划资助项目(No.2009Z1E2012);中山大学中山医学院学生论文出版基金资助项目

摘　　要：目的:比较姜黄素衍生物(B50)抑制不同辐射抗拒的同源鼻咽癌细胞CNE-2与CNE-2R作用的差异。方法:采用MTT法和克隆形成实验检测B50对细胞活力及增殖的影响,以流式细胞仪检测B50对细胞周期分布、细胞凋亡及线粒体膜电位的影响。结果:B50可抑制CNE-2R细胞的活力[24 h、48 h、72 h的IC50分别为(8.06±0.14)、(2.49±0.02)、(1.42±0.02)μmol/L],且呈时间浓度依赖性,其抑制作用明显优于对CNE-2细胞的作用[24 h、48 h、72 h的IC50分别为(8.18±0.12)μmol/L、(3.00±0.04)μmol/L、(1.75±0.01)μmol/L],P<0.01;同时B50抑制CNE-2R细胞增殖的作用明显优于对CNE-2的作用[48 h的IC50分别为(0.55±0.01)μmol/Lvs(0.82±0.01)μmol/L],P<0.01;药物作用48 h后,B50使CNE-2R细胞G2/M期的比例升高(7.1%→34.9%),明显优于CNE-2细胞(12.4%→35.7%)(P<0.01);B50作用于CNE-2R细胞24 h后早期凋亡率升高(3.7%→19.5%),明显优于CNE-2细胞(4.4%→14.8%)(P<0.01);B50作用24 h后,CNE-2R细胞线粒体膜电位下降了(43.17±3.11)%,明显优于对CNE-2细胞的降低率[(35.06±1.07)%](P<0.01)。结论:与CNE-2相比,B50能更显著地抑制辐射抗拒CNE-2R细胞的细胞活力及增殖能力,且更明显地调控细胞周期停滞于G2/M期,诱导细胞凋亡及降低线粒体膜电位。这些结果提示B50具有逆转辐射抗拒的潜在价值。AIM：To compare the effects of B50,a mono-carbonyl analogue of curcumin,on the prol iferation and apoptosis between homologous nasopharyngeal carcinoma cells CNE-2R and CNE-2 with different radioresistance.METHODS： The effects of B50 on cell viability and cell growth were detected by MTT assay and colony-forming experiment,respectively.The changes of cell cycle,apoptosi s and mitochondrial membrane potential（MMP） were determined by flow cytometry. RESULTS： B50 inhibited the cell viability of CNE-2R cells in a time-and dose-dependent ma nner with the IC50 of（8.06±0.14） μmol/L（24 h）,（2.49±0.02）μmol/L （48 h） and（1.42±0.02） μmol/L（72 h）,which was more effective than that in CNE-2 cells [with the IC50 of（8.18±0.12） μmol/L（24 h）,（3.00±0. 04） μmol/L（48 h） and（1.75±0.01） μmol/L（72 h）].The inhibitory effect of B50 on CNE-2R cell growth was more effective than that on CNE-2 cells [the IC 50 of 48 h was（0.55±0.01） μmol/L vs（0.82±0.01） μmol/L].Aft er treated with B50 for 48 h,the proportion of CNE-2R cells in G2/M stage was increased from 7.1% to 34.9%,which was better than that of CNE-2 cells（from 12.4% to 35.7%）.After treated with B50 for 24 h,the early apoptotic rate in CNE-2R cells was increased from 3.7% to 19.5%,which was better than that in CN E-2 cells（from 4.4% to 14.8%）,and the MMP in CNE-2R cells was decreased by（ 43.17±3.11）%,which was better than that in CNE-2 cells[（35.06±1.07）%]. CONCLUSION： B50 is more effective on inhibiting the cell viability and cell growth,blocking the cell cycle at G2/M stage,inducing apoptosis and decreasing MMP in CNE-2R cells than those in CNE-2 cells,indicating that B50 may enhance the radio-sens itivity of CNE-2R cells by blocking the cell cycle and inducing apoptosis throug h mitochondrial pathway.

关 键 词：鼻咽肿瘤 CNE-2细胞 CNE-2R细胞 姜黄素衍生物 辐射抗拒 

分 类 号：R739.63[医药卫生—肿瘤]