STYK1/NOK基因转染对肺腺癌细胞迁移和侵袭的影响  

Impact of STYK1/NOK gene transfection on the migration and invasion of lung adenocarcinoma cell line

作　　者：陈鹏[1] 张志培[1] 金河天[2] 刘涛[1] 李小飞[1] 

机构地区：[1]第四军医大学唐都医院胸外科,陕西西安710038 [2]沈阳军区第二零二医院放射治疗中心,辽宁沈阳110003

出　　处：《中华肿瘤防治杂志》2011年第8期564-567,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30973379);陕西省科学技术研究发展计划基金(2008K09-01)

摘　　要：目的:探讨STYK1/NOK基因对肺腺癌细胞迁移和侵袭的影响。方法:运用电穿孔仪将含STYK1/NOK全长基因的重组真核表达质粒(pcDNA3.1/NOK)转染入肺腺癌细胞系A549,筛选出稳定表达NOK蛋白的单克隆株A549-NOK。蛋白质印迹法检测转染前后细胞内NOK蛋白的表达效果。划痕实验、Transwell迁移实验检测转染前后细胞的迁移能力,Matrigel侵袭实验检测细胞的侵袭能力,MTT法绘制细胞生长曲线。结果:蛋白质印迹法显示,A549-NOK细胞中NOK蛋白表达量明显增高。划痕实验结果显示,A549-NOK细胞的迁移速度加快。在Transwell迁移实验中,A549-NOK组的穿膜细胞数较A549及空质粒对照组增多(95.7±9.5vs69.1±8.3、70.5±7.5),P值分别为0.003和0.004。Matrigel侵袭实验中A549-NOK组的穿膜细胞数较A549及空质粒对照组增多(67.8±6.7vs36.0±4.1、38.3±5.1),P值分别为0.000和0.001。MTT结果显示,A549-NOK细胞较A549和空质粒对照组细胞生长曲线上移。结论:STYK1/NOK基因能够促进肺腺癌细胞的迁移和侵袭,可能在肺癌的转移中发挥重要作用。OBJECTIVE： To investigate the impact of STYK1/NOK gene on the migration and invasion of lung adenocarcinoma cell line. METHODS： The recombinant STYK1/NOK eukaryotic expression plasmid was transfected into A549 cells with electroporation apparatus. The cloned strain A549-NOK with stable expression of NOK was screened, then the protein NOK was detected by Western blot. Cell migration was tested by scratch-wound assay and transwell migration assay. Cell invasion was tested by matrigel invasion assay. The cell growth curve was determined by MTT. RESULTS： Western blot showed the high expression level of NOK in A549-NOK cells. Scratch-wound assay showed the migrating speed of A549-NOK was faster than those of the controls. Transwell migration assay showed that the number of A549-NOK cells that passed the Transwell membrane was more than those of A549 and blank control cells （95.7±9.5 vs 69.1±8.3, 70.5±7.5, and P values were 0. 003 and 0. 004）. Mattrigel invasion assay showed that the number of A549-NOK cells that passed the Transwell membrane was more than those of A549 and blank control cells （67.8±6.7 vs 36.0±4.1, 38.3±5.1, and P values were 0. 000 and 0. 001）. The growth curve of A549-NOK cells was steeper than that of A549 and blank control cells. CONCLUSION： STYK1/NOK gene can improve the migration and invasion capabilities of line and may play an important lung cancer. human lung adenocarcinoma cell role in the metastasis of human

关 键 词：肺肿瘤 细胞系 肿瘤 基因 转染 细胞运动 肿瘤侵润 

分 类 号：R734.2[医药卫生—肿瘤]