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作 者:刘亮[1,2,3] 孟祥黔[2,3,4] 吴彩凤[2,3] 戴建军[2,3] 张廷宇[2,3] 顾晓龙[2,3,4] 吴志强[2,3,4] 谢一妮[2,3,4] 张树山[2,3] 张德福[2,3]
机构地区:[1]上海海洋大学生命与水产学院,上海201306 [2]上海市农业科学院畜牧兽医研究所,上海201106 [3]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [4]四川农业大学生命科学与理学院,雅安625014
出 处:《上海农业学报》2011年第2期45-48,共4页Acta Agriculturae Shanghai
基 金:上海市科学技术委员会重大科技攻关(生态崇明)(07DZ19603);国家863计划项目(2009AA101001);上海市农业科学院青年科技发展基金项目[农青科技2009(15)];上海市科技兴农推广项目(2010-3-2)资助
摘 要:对崇明白山羊精液采取3种不同离心处理去除精清,比较添加鹌鹑蛋黄和鸡蛋黄对精液冷冻保存效果的影响。结果表明:崇明白山羊精液冷冻保存中以1000 r/min离心5min去除精清后,添加鸡蛋黄组解冻后精子活力为50.48%,鹌鹑蛋黄组解冻后精子活力为42.98%。添加鸡蛋黄组的效果显著优于鹌鹑蛋处理组。The seminal plasma was removed from Chongming white goat sperm by 3 different centrifugation treatments and the effects of chicken and quail egg yolks added on the cryopreservation of Chongming white goat sperm were investigated.The results showed that after removing the seminal plasma by centrifugation at 1 000 r/min for 5 min,the sperm added with chicken egg yolk was 50.48% in motility after thawing,and the sperm added with quail egg yolk was 42.98%in motility after thawing, indicating that the effect of adding chicken egg yolk was significantly better than that of adding quail egg yolk in the cryopreservation of Chongming goat sperm.
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